H8 Biosimilar - Anti-5T4 Antibody - BSA and Azide free
Description
Recombinant humanized monoclonal antibody to 5T4.
Applications
ELISA, Flow Cytometry
Dilutions
ELISA: 1:5,000-10,000, Flow Cytometry: 1:100
Reactivity
Human
Host
Humanized
Clonality
Monoclonal
Isotype
IgG1
Conjugate
Unconjugated
Purification
Affinity chromatography.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Reconstitute with distilled sterile water.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
General Notes
Prior to reconstitution, centrifuge the vial at 5,000g for 3-5 minutes at room temperature. Reconstitute with appropriate volume of distilled sterile water to bring product to 1mg/ml concentration. After addition of distilled sterile water, mix by gentle tapping. Note: It is not recommended to vortex or vigorously pipette the sample.
5T4 protein is highly expressed on the surface of Expi293 cell membrane. Flow cytometry analysis with 15 µg/ml Anti-5T4 Humanized Antibody [H8 Biosimilar] - Azide free (A318817) on Expi293 cells transfected with human 5T4 (blue histogram) or Expi293 transfected with irrelevant protein (red histogram), and isotype control on Expi293 transfected with irrelevant protein (orange histogram).
ELISA plates were pre-coated with 2 µg/ml (100 µl/well) Recombinant Human 5T4 Protein (6xHis Tag) (A318304) which can bind Anti-5T4 Humanized Antibody [H8 Biosimilar] - Azide free (A318817) in a linear range of 0.64-16 µg/ml.
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