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Anti-GW182 Antibody (A17219)

Western Blot - Anti-GW182 Antibody (A17219) - Antibodies.com Immunofluorescence - Anti-GW182 Antibody (A17219) - Antibodies.com
$335
Product Datasheet

Rabbit polyclonal antibody to GW182 for WB and ICC/IF.

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$40 to United States
Lead Time
5-8 Business Days

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Name
Anti-GW182 Antibody
Description
Rabbit polyclonal antibody to GW182.
Applications
WB, ICC/IF
Dilutions
WB: 1:500-1:1,000, ICC/IF: 1:50-1:100
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 950-1050 of human TNRC6A (NP_055309.2).
Sequence
QDIVGSWGIPPATGKPPGTGWLGGPIPAPAKEEEPTGWEEPSPESIRRKMEIDDGTSAWGDPSKYNYKNVNMWNKNVPNGNSRSDQQAQVHQLLTPASAIS
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
180 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
CAG repeat protein 26, CAGH26, DKFZp666E117, EDIE, EMSY interactor protein, FLJ22043, Glycine tryptophan protein of 182 kDa, Glycine-tryptophan protein of 182 kDa, GW1, GW182 autoantigen, Hypothetical protein DKFZp566M143, KIAA1460, MGC75384, OTTHUMP00000122485, OTTHUMP00000195164, OTTHUMP00000195165, Protein GW1, TNR6A_HUMAN, TNRC 6A, TNRC6, Tnrc6a, Trinucleotide repeat containing 6A, Trinucleotide repeat containing gene 6A protein, Trinucleotide repeat-containing gene 6A protein
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-GW182 Antibody (A17219) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-GW182 Antibody (A17219)
Western blot analysis of extracts of various cell lines, using Anti-GW182 Antibody (A17219) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Immunofluorescence - Anti-GW182 Antibody (A17219) - Antibodies.comEnlarge Image
Figure 2: Immunofluorescence - Anti-GW182 Antibody (A17219)
Immunofluorescence analysis of U2OS cells using Anti-GW182 Antibody (A17219) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).
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