Anti-GSK3 alpha + beta (phospho Tyr279 + Tyr216) Antibody (A95108)

Name: Anti-GSK3 alpha + beta (phospho Tyr279 + Tyr216) Antibody
Brand: Antibodies.com
SKU: A95108
Price: 185.0 USD
Availability: InStock

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$185

Rabbit polyclonal antibody to GSK3 alpha + beta (phospho Tyr279 + Tyr216) for WB, IHC and ELISA.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Specifications

Name

Anti-GSK3 alpha + beta (phospho Tyr279 + Tyr216) Antibody

Description

Rabbit polyclonal antibody to GSK3 alpha + beta (phospho Tyr279 + Tyr216).

Specificity

This antibody detects endogenous levels of GSK3 alpha/beta only when phosphorylated at Tyr279/216.

Applications

WB, IHC, ELISA

Dilutions

IHC: 1:50-1:100, ELISA: 1:5000

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide derived from human GSK3 alpha/beta around the phosphorylation site of Tyr279/216 (amino acids 246-295).

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.

Molecular Weight

50kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (8)

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Figure 1: Western Blot - Anti-GSK3 alpha + beta (phospho Tyr279 + Tyr216) Antibody (A95108)

Western blot analysis of lysates from HeLa cells using Anti-GSK3 alpha + beta (phospho Tyr279 + Tyr216) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.

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Figure 2: Immunohistochemistry - Anti-GSK3 alpha + beta (phospho Tyr279 + Tyr216) Antibody (A95108)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Anti-GSK3 alpha + beta (phospho Tyr279 + Tyr216) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.

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Figure 3: Western Blot - Anti-GSK3 alpha + beta (phospho Tyr279 + Tyr216) Antibody (A95108)

Western blot analysis of various cells using Anti-GSK3 alpha + beta (phospho Tyr279 + Tyr216) Antibody at 1:1,000 (4°C overnight). Goat Anti-Rabbit IgG (IRDye 800) was used as a secondary at 1:5,000 (25°C, 1 hour).

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Figure 4: Western Blot - Anti-GSK3 alpha + beta (phospho Tyr279 + Tyr216) Antibody (A95108)

Western blot analysis of various cells using Anti-GSK3 alpha + beta (phospho Tyr279 + Tyr216) Antibody.

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