Anti-GSK3 alpha Antibody [ARC0462] (A307767)

Name: Anti-GSK3 alpha Antibody [ARC0462]
Brand: Antibodies.com
SKU: A307767
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC0462] antibody to GSK3 alpha for WB and IP.

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Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Tags:

Knockout Validated Antibody

Specifications

Name

Anti-GSK3 alpha Antibody [ARC0462]

Description

Rabbit monoclonal [ARC0462] antibody to GSK3 alpha.

Applications

WB, IP

Dilutions

WB: 1:500-1:2,000, IP: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human GSK3a (P49840).

Sequence

MSGGGPSGGGPGGSGRARTSSFAEPGGGGGGGGGGPGGSASGPGGTGGGKASVGAMGGGVGASSSGGGPGGSGGGGSGGPGAGTSFPPPGVKLGRDSGKV

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0462

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

51 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

DKFZp686D0638, Glycogen synthase kinase 3 alpha, Glycogen synthase kinase-3 alpha, GSK 3 alpha, GSK 3A, GSK-3 alpha, Gsk3a, GSK3alpha, GSK3A_HUMAN, Serine/threonine protein kinase GSK3A

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-GSK3 alpha Antibody [ARC0462] (A307767)

Western blot analysis of extracts from wild type (WT) and GSK3alpha knockout (KO) HeLa cells, using Anti-GSK3 alpha Antibody [ARC0462] (A307767) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 2: Western Blot - Anti-GSK3 alpha Antibody [ARC0462] (A307767)

Western blot analysis of extracts of Rat lung, using Anti-GSK3 alpha Antibody [ARC0462] (A307767) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Figure 3: Western Blot - Anti-GSK3 alpha Antibody [ARC0462] (A307767)

Western blot analysis of extracts of Mouse brain, using Anti-GSK3 alpha Antibody [ARC0462] (A307767) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 4: Western Blot - Anti-GSK3 alpha Antibody [ARC0462] (A307767)

Immunoprecipitation analysis of 300µg extracts of HeLa cells using 3µg of Anti-GSK3 alpha Antibody [ARC0462] (A307767). This Western blot was performed on the immunoprecipitate using Anti-GSK3 alpha Antibody [ARC0462] (A307767) at a dilution of 1:1000.

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