Anti-GSDMD Antibody (A256242)

Western blot analysis of extracts from B16F10 cells, using GSDMD Antibody. The lane on the left was treated with blocking peptide.

AF4012 staining Hela(LPS treated 4h) by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AF4012 1:200) and mouse anti-beta tubulin Ab(T0023 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI(blue).

Western blot analysis of extracts from various samples, using GSDMD Antibody. Lane 1: C6(TPA + LPS treatment)lysates treated with blocking peptide; Lane 2: C6(TPA treatment) lysates; Lane 3: HepG2(TPA treatment) lysates; Lane 4: C6(TPA and LPS treatment) lysates; Lane 5:HepG2(TPA and LPS treatment) lysates;