Anti-GRP94 Antibody (A84222)

Name: Anti-GRP94 Antibody
Brand: Antibodies.com
SKU: A84222
Price: 480.0 USD
Availability: InStock

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$480

Goat polyclonal antibody to GRP94 for ELISA, WB, IHC, IF and Flow Cytometry.

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Delivery

Freight Charges

$40 to United States

Lead Time

3-5 Business Days

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+1 (314) 370-6046

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ER Marker

Specifications

Name

Anti-GRP94 Antibody

Description

Goat polyclonal antibody to GRP94.

Applications

ELISA, WB, IHC, IF, Flow Cytometry

Dilutions

ELISA: 1:32,000, WB: 0.1-0.3µg/ml, IHC: 3-5µg/ml, IF: 10µg/ml

Reactivity

Human, Mouse

Immunogen

Synthetic peptide corresponding to the internal region of human GRP94.

Sequence

C-KEGVKFDESEKTKE

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Molecular Weight

100 kDa

Predicted MW

92.5kDa

Product Form

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

94 kDa glucose regulated protein, 94 kDa glucose-regulated protein, ECGP, Endoplasmin, Endothelial cell (HBMEC) glycoprotein, ENPL_HUMAN, Glucose regulated protein 94kDa, gp96, gp96 homolog, GRP 94, GRP-94, Heat shock protein 90 kDa beta member 1, heat shock protein 90kDa beta (Grp94), member 1, Heat shock protein, 90 kDa, beta, 1, HSP90B1, Stress inducible tumor rejection antigen GP96, TRA1, tumor rejection antigen (gp96) 1, Tumor rejection antigen 1, Tumor rejection antigen gp96, Tumor rejection antigen-1 (gp96)

Isotype Controls

Goat IgG (A121671)

Suitable Secondaries

See all Anti-Goat IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (7)

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Figure 1: Western Blot - Anti-GRP94 Antibody (A84222)

GRP94 expression in U2OS (A), HEK293 (B), HeLa (C), and A549 (D) cell lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-GRP94 Antibody (A84222) at 0.1µg/ml (A-C) or 0.3µg/ml (D) and detected by chemiluminescence.

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Figure 2: Western Blot - Anti-GRP94 Antibody (A84222)

GRP94 expression in NIH3T3 cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-GRP94 Antibody (A84222) at 0.1µg/ml and detected by chemiluminescence.

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Figure 3: Immunofluorescence - Anti-GRP94 Antibody (A84222)

GRP94 expression in A431 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-GRP94 Antibody (A84222) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic and ER staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Figure 4: Immunofluorescence - Anti-GRP94 Antibody (A84222)

GRP94 expression in HeLa cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-GRP94 Antibody (A84222) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Cytoplasmic and ER staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.