Anti-GRP78 Antibody [1H11-1H7] (A305099)

Name: Anti-GRP78 Antibody [1H11-1H7]
Brand: Antibodies.com
SKU: A305099
Price: 450.0 USD
Availability: InStock

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$450

Mouse monoclonal [1H11-1H7] antibody to GRP78 for WB and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Ribosomal Marker

Specifications

Name

Anti-GRP78 Antibody [1H11-1H7]

Description

Mouse monoclonal [1H11-1H7] antibody to GRP78.

Applications

WB, ICC/IF

Dilutions

WB: 1:2,000, ICC/IF: 1:100

Reactivity

Human, Mouse, Rat, Bovine, Fungi, Hamster, Monkey, Rabbit, Xenopus

Immunogen

His-tagged human GRP78.

Host

Mouse

Clonality

Monoclonal

Clone ID

1H11-1H7

Isotype

IgG2b

Conjugate

Unconjugated

Purification

Protein G purification.

Concentration

1 mg/ml

Molecular Weight

~78 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

78 kDa glucose regulated protein, 78 kDa glucose-regulated protein, AL022860, AU019543, BIP, D2Wsu141e, D2Wsu17e, Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78, Endoplasmic reticulum lumenal Ca2+ binding protein grp78, Epididymis secretory sperm binding protein Li 89n, FLJ26106, Glucose Regulated Protein 78kDa, GRP 78, GRP-78, GRP78 BiP, GRP78_HUMAN, Heat shock 70 kDa protein 5, Heat Shock 70kDa Protein 5, Heat shock protein family A (Hsp70) member 5, HEL S 89n, Hsce70, HSPA 5, HSPA5, Immunoglobulin Heavy Chain Binding Protein, Immunoglobulin heavy chain-binding protein, mBiP, MIF2, Sez7

Isotype Controls

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Immunofluorescence - Anti-GRP78 Antibody [1H11-1H7] (A305099)

Immunocytochemistry/Immunofluorescence analysis of human heat shocked cervical cancer cell line (HeLa), fixed in 2% formaldehyde for 20 minutes at room temperature, using Anti-GRP78 Antibody [1H11-1H7] (A305099), at 1:100 for 12 hours at 4°C. The secondary antibody used was FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at room temperature. Localization: Endoplasmic reticulum lumen. Melanosome. Magnification: 20x.(A) DAPI (blue) nuclear stain. (B) Anti-GRP78 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.

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Figure 2: Western Blot - Anti-GRP78 Antibody [1H11-1H7] (A305099)

Western blot analysis of human cell lysates showing detection of GRP78 protein using Anti-GRP78 Antibody [1H11-1H7] (A305099) at 1:1,000.

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Figure 3: Immunofluorescence - Anti-GRP78 Antibody [1H11-1H7] (A305099)

Immunocytochemistry/Immunofluorescence analysis of human heat shocked cervical cancer cell line (HeLa), fixed in 2% formaldehyde for 20 minutes at room temperature, using Anti-GRP78 Antibody [1H11-1H7] (A305099), at 1:100 for 12 hours at 4°C. The secondary antibody used was FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at room temperature. Localization: Endoplasmic reticulum lumen. Melanosome. Magnification: 100x.(A) DAPI (blue) nuclear stain. (B) Anti-GRP78 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.

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