Anti-GRK2 Antibody (A13580)

Name: Anti-GRK2 Antibody
Brand: Antibodies.com
SKU: A13580
Price: 470.0 USD
Availability: InStock

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$470

Rabbit polyclonal antibody to GRK2 for WB and IHC.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Knockout Validated Antibody

Specifications

Name

Anti-GRK2 Antibody

Description

Rabbit polyclonal antibody to GRK2.

Applications

WB, IHC

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 440-689 of human GRK2 (NP_001610.2).

Sequence

LGRGAQEVKESPFFRSLDWQMVFLQKYPPPLIPPRGEVNAADAFDIGSFDEEDTKGIKLLDSDQELYRNFPLTISERWQQEVAETVFDTINAETDRLEARKKAKNKQLGHEEDYALGKDCIMHGYMSKMGNPFLTQWQRRYFYLFPNRLEWRGEGEAPQSLLTMEEIQSVEETQIKERKCLLLKIRGGKQFILQCDSDPELVQWKKELRDAYREAQQLVQRVPKMKNKPRSPVVELSKVPLVQRGSANGL

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

80 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

ADRBK1, Adrenergic beta receptor kinase 1, ARBK1_HUMAN, BARK, BARK1, Beta adrenergic receptor kinase 1, Beta ARK 1, Beta ARK1, Beta-adrenergic receptor kinase 1, Beta-ARK-1, FLJ16718, G protein coupled receptor kinase 2, G-protein coupled receptor kinase 2

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-GRK2 Antibody (A13580)

Western blot analysis of extracts of various cell lines, using Anti-GRK2 Antibody (A13580) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST.

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Figure 2: Western Blot - Anti-GRK2 Antibody (A13580)

Western blot analysis of extracts from normal (control) and GRK2 knockout (KO) 293T cells, using Anti-GRK2 Antibody (A13580) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 3: Immunohistochemistry - Anti-GRK2 Antibody (A13580)

Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-GRK2 Antibody (A13580) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-GRK2 Antibody (A13580)

Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using Anti-GRK2 Antibody (A13580) at a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.