Anti-GRF-1 (phospho Tyr1105) Antibody (A93973)

Name: Anti-GRF-1 (phospho Tyr1105) Antibody
Brand: Antibodies.com
SKU: A93973
Price: 185.0 USD
Availability: InStock

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$185

Rabbit polyclonal antibody to GRF-1 (phospho Tyr1105) for WB, IHC and ELISA.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-GRF-1 (phospho Tyr1105) Antibody

Description

Rabbit polyclonal antibody to GRF-1 (phospho Tyr1105).

Specificity

This antibody detects endogenous levels of GRF-1 only when phosphorylated at Tyr1105.

Applications

WB, IHC, ELISA

Dilutions

WB: 1:500-1:1000, ELISA: 1:40000

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide derived from human GRF-1 around the phosphorylation site of Tyr1105 (amino acids 1071-1120).

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.

Molecular Weight

172kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

ARHGAP35, GAP associated protein p190, Glucocorticoid receptor DNA binding factor 1, Glucocorticoid receptor DNA-binding factor 1, Glucocorticoid receptor repression factor 1, GRF 1, GRF-1, GRF1, GRLF 1, GRLF1_HUMAN, KIAA1722, MGC10745, p190 A, p190-A, P190A, P190A, rat, homolog of, p190ARhoGAP, p190RhoGAP, Rho GAP p190A, Rho GTPase-activating protein 35

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-GRF-1 (phospho Tyr1105) Antibody (A93973)

Western blot analysis of lysates from 293 cells treated with EGF 200ng/ml 30' using Anti-GRF-1 (phospho Tyr1105) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.

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Figure 2: Immunohistochemistry - Anti-GRF-1 (phospho Tyr1105) Antibody (A93973)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Anti-GRF-1 (phospho Tyr1105) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.

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Figure 3: Western Blot - Anti-GRF-1 (phospho Tyr1105) Antibody (A93973)

Western blot analysis of 3T3 cells using Anti-GRF-1 (phospho Tyr1105) Antibody.

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Figure 4: Immunohistochemistry - Anti-GRF-1 (phospho Tyr1105) Antibody (A93973)

Immunohistochemical analysis of paraffin-embedded human brain using Anti-GRF-1 (phospho Tyr1105) Antibody 1:100 (4°C overnight). The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.

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