Anti-GRB2 Antibody [ARC0430] (A309206)

Name: Anti-GRB2 Antibody [ARC0430]
Brand: Antibodies.com
SKU: A309206
Price: 470.0 USD
Availability: InStock

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$470

Rabbit monoclonal [ARC0430] antibody to GRB2 for WB, IHC, ICC/IF and IP.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Plasma Membrane Marker

Specifications

Name

Anti-GRB2 Antibody [ARC0430]

Description

Rabbit monoclonal [ARC0430] antibody to GRB2.

Applications

WB, IHC, ICC/IF, IP

Dilutions

WB: 1:500-1:2,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 118-217 of human GRB2 (P62993).

Sequence

YFLWVVKFNSLNELVDYHRSTSVSRNQQIFLRDIEQVPQQPTYVQALFDFDPQEDGELGFRRGDFIHVMDNSDPNWWKGACHGQTGMFPRNYVTPVNRNV

Host

Rabbit

Clonality

Monoclonal

Clone ID

ARC0430

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

25 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Abundant SRC homology, Adapter protein GRB2, ASH, Ash protein, EGFRBP GRB2, Epidermal growth factor receptor binding protein, Epidermal growth factor receptor binding protein GRB2, GRB 2, GRB2 adapter protein, GRB2_HUMAN, Grb3 3, Growth factor receptor bound protein 2, Growth factor receptor bound protein 3, Growth factor receptor-bound protein 2, HT027, MST084, MSTP084, NCKAP2, OTTHUMP00000166096, OTTHUMP00000166097, OTTHUMP00000166098, Protein ash, SEM5, SH2/SH3 adapter GRB2

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (6)

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Figure 1: Western Blot - Anti-GRB2 Antibody [ARC0430] (A309206)

Western blot analysis of extracts of various cell lines, using Anti-GRB2 Antibody [ARC0430] (A309206) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 5s.

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Figure 2: Immunohistochemistry - Anti-GRB2 Antibody [ARC0430] (A309206)

Immunohistochemistry analysis of paraffin-embedded mouse spleen using Anti-GRB2 Antibody [ARC0430] (A309206) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 3: Immunohistochemistry - Anti-GRB2 Antibody [ARC0430] (A309206)

Immunohistochemistry analysis of paraffin-embedded rat spleen using Anti-GRB2 Antibody [ARC0430] (A309206) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Figure 4: Immunohistochemistry - Anti-GRB2 Antibody [ARC0430] (A309206)

Immunohistochemistry analysis of paraffin-embedded human lung cancer using Anti-GRB2 Antibody [ARC0430] (A309206) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.