Anti-Granulocytes Antibody [6D10] (A279476)

Anti-Granulocytes Antibody [6D10]

Mouse monoclonal [6D10] antibody to Granulocytes.

This antibody recognises a ~60 kDa antigen on porcine granulocytes of the neutrophil lineage, acting as a reliable tool for their analysis and isolation, without contamination from other cells.Expression of the antigen recognized by clone 6D10 decreases from the immature promyelocytes, through myelocytes and metamyelocytes, to the mature neutrophils, thereby enabling the identification of neutrophil developmental stages. Furthermore, use of clone 6D10 in conjunction with clone 2B2 (MCA2600), allows for the discrimination and characterisation of different porcine granulocyte lineages and also their developmental stages: 6D10-2B2- early myeloid precursors, 6D10+2B2- immature neutrophils, 6D10+2B2+ mature neutrophils and 6D10-2B2+ mature eosinophils and basophils. Mouse anti Pig Granulocytes antibody, clone 6D10 has been shown as suitable for use on cytospins (Pérez et al. 2007).

WB, IP, Flow Cytometry

Flow Cytometry: 1:25 - 1:200, Use 10µl of the suggested working dilution to label 1x10⁶ cells in 100µl.

Porcine

Porcine bone marrow haematopoietic cells (BMHC).

Mouse

Monoclonal

6D10

IgG2a

1 mg/ml

Approximately 60 kDa in porcine BMHC cell lysates.

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Pig Granulocytes antibody, clone 6D10 recognizes a ~60 kDa antigen on porcine granulocytes of the neutrophil lineage, acting as a reliable tool for their analysis and isolation, without contamination from other cells.Expression of the antigen recognized by clone 6D10 decreases from the immature promyelocytes, through myelocytes and metamyelocytes, to the mature neutrophils, thereby enabling the identification of neutrophil developmental stages. Furthermore, use of clone 6D10 in conjunction with clone 2B2 (MCA2600), allows for the discrimination and characterisation of different porcine granulocyte lineages and also their developmental stages: 6D10-2B2- early myeloid precursors, 6D10+2B2- immature neutrophils, 6D10+2B2+ mature neutrophils and 6D10-2B2+ mature eosinophils and basophils.Mouse anti Pig Granulocytes antibody, clone 6D10 has been shown as suitable for use on cytospins (Pérez et al. 2007).