Anti-Glutathione Peroxidase 4 Antibody (A13753)

Name: Anti-Glutathione Peroxidase 4 Antibody
Brand: Antibodies.com
SKU: A13753

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This product is discontinued

Anti-Glutathione Peroxidase 4 Antibody (A13753) has been discontinued and is no longer available.

View all Glutathione Peroxidase 4 Antibodies.

Specifications

Name

Anti-Glutathione Peroxidase 4 Antibody

Description

Rabbit polyclonal antibody to Glutathione Peroxidase 4.

Applications

WB, ICC/IF

Dilutions

WB: 1:500-1:1,000, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 74-197 of GPX4 (NP_002076.2).

Sequence

GKTEVNYTQLVDLHARYAECGLRILAFPCNQFGKQEPGSNEEIKEFAAGYNVKFDMFSKICVNGDDAHPLWKWMKIQPKGKGILGNAIKWNFTKFLIDKNGCVVKRYGPMEEPLVIEKDLPHYF

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

22 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

EC:1.11.1.9, GPx-4, GSHPx-4, PHGPx, Phospholipid hydroperoxide glutathione peroxidase GPX4

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (5)

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Western Blot - Anti-Glutathione Peroxidase 4 Antibody (A13753)

Western blot analysis of extracts of various cell lines, using Anti-Glutathione Peroxidase 4 Antibody (A13753) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

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Immunofluorescence - Anti-Glutathione Peroxidase 4 Antibody (A13753)

Immunofluorescence analysis of A-549 cells using Anti-Glutathione Peroxidase 4 Antibody (A13753) at a dilution of 1:200 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Immunofluorescence - Anti-Glutathione Peroxidase 4 Antibody (A13753)

Immunofluorescence analysis of C6 cells using Anti-Glutathione Peroxidase 4 Antibody (A13753) at a dilution of 1:200 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Immunofluorescence - Anti-Glutathione Peroxidase 4 Antibody (A13753)

Immunofluorescence analysis of NIH/3T3 cells using Anti-Glutathione Peroxidase 4 Antibody (A13753) at a dilution of 1:200 (40x lens). DAPI was used to stain the cell nuclei (blue).