Goat Anti-Mouse IgG H&L Antibody (AP), Cross-Absorbed (A282165)

Goat Anti-Mouse IgG H&L Antibody (AP), Cross-Absorbed

Goat anti-mouse IgG H&L (AP) secondary antibody.

Goat

Mouse

This antibody recognises mouse IgG and light chains common to other mouse immunoglobulin classes.Goat anti Mouse IgG has been cross-adsorbed using human, bovine, porcine, equine, lapine and chicken immunoabsorbants to remove cross-reactive antibodies. Less than 0.1% cross reactivity was detected to human, bovine, porcine, equine, caprine, lapine and chicken IgG by immunoelectrophoresis and ELISA.Goat anti Mouse IgG antibody is highly recommended for use as a secondary antibody with human and veterinary samples. Goat anti Mouse IgG antibody has been used successfully as a secondary detection reagent in combination with mouse clone CC327 for the detection of TNFα and mouse clone 8M6 for the detection of interleukin-8 in bovine respiritory syncitial virus infected, neonatal ovine lung tissue by immunohistochemistry.

WB, ELISA

ELISA: 1 µg/ml - 10 µg/ml, Use 50 µl of the suggested working dilution to label 1x10⁶ cells in 100µl

Whole mouse IgG.

IgG

AP

Liquid

500 µg/ml

Supplied in 50 mM HEPES, pH 7.1, with 0.1M NaCl, 1mM MgCl₂, 0.1mM ZnCl₂, 0.2% BSA, and 0.09% Sodium Azide.

Store undiluted at 4°C. Do not freeze! Should this product contain a precipitate we recommend microcentrifugation before use.

Goat anti Mouse IgG antibody recognizes mouse IgG and light chains common to other mouse immunoglobulin classes.Goat anti Mouse IgG has been cross-adsorbed using human, bovine, porcine, equine, lapine and chicken immunoabsorbants to remove cross-reactive antibodies. Less than 0.1% cross reactivity was detected to human, bovine, porcine, equine, caprine, lapine and chicken IgG by immunoelectrophoresis and ELISA.Goat anti Mouse IgG antibody is highly recommended for use as a secondary antibody with human and veterinary samples. Goat anti Mouse IgG antibody has been used successfully as a secondary detection reagent in combination with mouse clone CC327 for the detection of TNFa and mouse clone 8M6 for the detection of interleukin-8 in bovine respiritory syncitial virus infected, neonatal ovine lung tissue by immunohistochemistry (Redondo et al. 2013).

This product is for research use only. It is not intended for diagnostic or therapeutic use.