Goat F(ab')2 Anti-Human IgG F(ab')2 Antibody (AP), Cross-Adsorbed (A301275)

Goat F(ab')2 anti-human IgG F(ab')2 (AP) secondary antibody for WB, IHC, ICC and ELISA.

Alternative Formats and ConjugatesAlternative Formats

Freight Charges
Lead Time
5-8 Business Days
Telephone
+1 (314) 370-6046
Mon - Fri, 8am - 4pm AST
Email
orders@antibodies.com
Name
Goat F(ab')2 Anti-Human IgG F(ab')2 Antibody (AP), Cross-Adsorbed
Description
Goat F(ab')2 anti-human IgG F(ab')2 (AP) secondary antibody.
Host Species
Goat
Target Species
Human
Applications
WB, IHC, ICC, ELISA
Recommended Dilutions
ELISA: 1:2,500-1:25,000, ICC: 1:100-1:1,000, IHC: 1:100-1:1,000, WB: 1:2,500-1:25,000
Clonality
Polyclonal
Isotype
IgG
Conjugate

AP

Product Form
Liquid
Concentration
0.5 mg/ml
Purity
Antigen affinity purification.
Purification
Antigen affinity purification.
Formulation
Supplied in 50 mM HEPES, pH 7.1, with 0.1 M Sodium Chloride, 1 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 0.2% BSA, and 0.09% Sodium Azide.
Storage
Store in the dark at 4°C. Avoid prolonged exposure to light. Do not freeze!
General Notes
Antiserum was solid phase adsorbed to ensure specificity, and subsequently cross-adsorbed using mouse and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion, and Fc fragments and whole IgG molecules were removed. F(ab')2 fragments were then conjugated to alkaline phosphatase (AP).
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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