Goat F(ab')2 Anti-Human IgG Fc Antibody (AP), Cross-Adsorbed (A301265)

Goat F(ab')2 Anti-Human IgG Fc Antibody (AP), Cross-Adsorbed

Goat F(ab')2 anti-human IgG Fc (AP) secondary antibody.

Goat

Human

WB, IHC, ICC, ELISA

WB: 1:2,500-1:25,000, IHC: 1:100-1:1,000, ICC: 1:100-1:1,000, ELISA: 1:2,500-1:25,000

Polyclonal

IgG

AP

0.5 mg/ml

Antigen affinity purification.

Antigen affinity purification.

Supplied in 50 mM HEPES, pH 7.1, with 0.1 M Sodium Chloride, 1 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 0.2% BSA, and 0.09% Sodium Azide.

Store in the dark at 4°C. Avoid prolonged exposure to light. Do not freeze!

Antiserum was solid phase adsorbed to ensure class specificity, and subsequently cross-adsorbed using mouse and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion, and Fc fragments and whole IgG molecules were removed. F(ab')2 fragments were then conjugated to alkaline phosphatase (AP).

This product is for research use only. It is not intended for diagnostic or therapeutic use.