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Anti-Glypican 3 Antibody (A13764)

Western Blot - Anti-Glypican 3 Antibody (A13764) - Antibodies.com Western Blot - Anti-Glypican 3 Antibody (A13764) - Antibodies.com
$335
Product Datasheet

Rabbit polyclonal antibody to Glypican 3 for WB and Flow Cytometry.

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$40 to United States
Lead Time
5-8 Business Days

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Name
Anti-Glypican 3 Antibody
Description
Rabbit polyclonal antibody to Glypican 3.
Applications
WB, Flow Cytometry
Dilutions
WB: 1:500-1:1,000, Flow Cytometry: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 481-580 of human Glypican 3 (GPC3) (NP_004475.1).
Sequence
GRVLDKNLDEEGFESGDCGDDEDECIGGSGDGMIKVKNQLRFLAELAYDLDVDDAPGNSQQATPKDNEISTFHNLGNVHSPLKLLTSMAISVVCFFFLVH
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
66 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
DGSX, Glypican proteoglycan 3, Glypican-3 [Precursor], Gpc3, GPC3_HUMAN, GTR2 2, GTR2-2, Heparan sulphate proteoglycan, Intestinal protein OCI 5, Intestinal protein OCI-5, MXR7, OCI 5, OCI-5, OCI5, SDYS, Secreted glypican-3, SGB, SGBS, SGBS1
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-Glypican 3 Antibody (A13764) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-Glypican 3 Antibody (A13764)
Western blot analysis of extracts of various cell lines, using Anti-Glypican 3 Antibody (A13764) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
Western Blot - Anti-Glypican 3 Antibody (A13764) - Antibodies.comEnlarge Image
Figure 2: Western Blot - Anti-Glypican 3 Antibody (A13764)
Western blot analysis of various lysates, using Anti-Glypican 3 Antibody (A13764) at 1:800 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Publishing research using Anti-Glypican 3 Antibody (A13764)? Please let us know so that we can list the citation on this page.

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