Western Blot - Anti-Glucose 6 Phosphate Isomerase Antibody [ARC0997] (A306804)
Western blot analysis of HeLe, using Anti-Glucose 6 Phosphate Isomerase Antibody [ARC0997] (A306804) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.
Western Blot - Anti-Glucose 6 Phosphate Isomerase Antibody [ARC0997] (A306804)
Western blot analysis of extracts from wild type(WT) and glucose-6-phosphateisomerase knockdown (KD) 293T(KD) cells, using Anti-Glucose 6 Phosphate Isomerase Antibody [ARC0997] (A306804) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 60s.
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