Mouse monoclonal [5E4] antibody to Glucocorticoid Receptor.
Specificity
This antibody recognises the human glucocorticoid receptor, also known as Nuclear receptor subfamily 3 group C member 1 (NR3C1), a 777 amino acid receptor bearing 3 distinct functional domains, an N-terminal modulating domain, a DNA binding domain and a C-terminal steroid binding domain. The human glucocorticoid receptor is located either in the cytoplasm of cells, prior to ligand binding associated with certain heat shock proteins , or the nucleus following binding to ligand. Mutations in the glucocorticoid receptor gene can lead to familial glucocorticoid resistance, characterized by elevated plasma cortisol levels. Mouse anti human glucocorticoid receptor antibody, clone 5E4 has been demonstrated to cross-react with the murine glucocorticoid receptor
Applications
Flow Cytometry
Dilutions
FC 1: Neat - 1:10, Use 10µl of the suggested working dilution to label 1x106 cells in 100µl.
Reactivity
Human, Mouse
Immunogen
A peptide corresponding to amino acids 150-176 of human GCR linked to human thyroglobulin.
Sequence
APTEKEFPKTHSDVSSEQQHLKGQTGT
Host
Mouse
Clonality
Monoclonal
Clone ID
5E4
Isotype
IgG1
Conjugate
Unconjugated
Purification
Affinity chromatography of tissue culture supernatant.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.1% BSA and 0.1% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Mouse anti Human Glucocorticoid Receptor antibody, clone 5E4 recognizes the human glucocorticoid receptor, also known as Nuclear receptor subfamily 3 group C member 1 (NR3C1), a 777 amino acid receptor bearing 3 distinct functional domains, an N-terminal modulating domain, a DNA binding domain and a C-terminal steroid binding domain. The human glucocorticoid receptor is located either in the cytoplasm of cells, prior to ligand binding associated with certain heat shock proteins (Kino et al. 2009), or the nucleus following binding to ligand (Werb et al. 1978).Mutations in the glucocorticoid receptor gene can lead to familial glucocorticoid resistance, characterized by elevated plasma cortisol levels (Malchoff et al. 1993).Mouse anti human glucocorticoid receptor antibody, clone 5E4 has been demonstrated to cross-react with the murine glucocorticoid receptor (Bergquist et al. 2014)
Synonyms
GCCR, GCR, GCRST, GCR_HUMAN, glucocorticoid nuclear receptor variant 1, GR, GRL, Grl1, nr3c1, Nuclear receptor subfamily 3 group C member 1, nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)