Anti-GLG1 Antibody [GLG1/970] (CF® 647) (A251133)

This product is discontinued

Anti-GLG1 Antibody [GLG1/970] (CF® 647) (A251133) has been discontinued and is no longer available.

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Name
Anti-GLG1 Antibody [GLG1/970] (CF® 647)
Description
Mouse monoclonal [GLG1/970] antibody to GLG1 (CF® 647).
Specificity
This antibody recognizes a protein of 134kDa, which binds fibroblast growth factor and E-selectin (cell-adhesion lectin on endothelial cells mediating the binding of neutrophils). Fucosylation is essential for binding to E-selectin. It contains sialic acid residues and 16 Cys-rich GLG1 repeats. Highest levels are expressed in pancreas, skeletal muscle, placenta, heart, testis and ovary. It is also found in the kidney, liver, lung and brain. It is expressed in both adult and fetal tissues. This antibody can be used to stain the Golgi complex in cell or tissue preparations and can be used as a Golgi marker in subcellular fractions. It produces a diffuse staining pattern of the Golgi zone in normal and malignant cells. This antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. The Golgi apparatus is an organelle present in all eukaryotic cells that forms a part of the endomembrane system. The primary function of the Golgi apparatus is to process and package macromolecules synthesized by the cell for exocytosis or use within the cell. The Golgi is made up of a stack of flattened, membrane-bound sacs known as cisternae, with three functional regions: the cis face, medial region and trans face. Each region consists of various enzymes that selectively modify the macromolecules passing though them, depending on where they are destined to reside. Several spherical vesicles that have budded off of the Golgi are present surrounding the main cisternae. The Golgi tends to be more pronounced and numerous in cells that make and secrete many substances such as plasma B cells.
Applications
Flow Cytometry, IF
Dilutions
Flow Cytometry: 5 µl / million cells or 5 µl / 100 µl whole blood, IF: 1:50-1:100
Reactivity
Human
Cross Reactivity
This antibody does not cross react with Mouse or Rat.
Immunogen
Golgi fraction from human liver cells.
Host
Mouse
Clonality
Monoclonal
Clone ID
GLG1/970
Isotype
IgG1
Light Chains
kappa
Conjugate

CF® 647

Excitation: 650nm, Emission: 665nm

Purification
Protein A/G chromatography.
Concentration
100 µg/ml
Molecular Weight
134 kDa
Product Form
Liquid
Formulation
Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.
Storage
Store at 4-8°C. Antibody is stable for 24 months.
Synonyms
AI593353, AW537898, CFR 1, CFR-1, CFR1, Cys -rich FGF Receptor, Cysteine rich fibroblast growth factor receptor, Cysteine-rich fibroblast growth factor receptor, DKFZp686L08213, E selectin ligand 1, E-selectin ligand 1, ESL 1, ESL-1, ESL1, FLJ23319, FLJ23967, FLJ32797, Golgi apparatus protein 1, Golgi Complex, golgi glycoprotein 1, Golgi sialoglycoprotein MG 160, Golgi sialoglycoprotein MG-160, GSLG1_HUMAN, MG 160, MG160, Mouse, homolog of, ESL1, Selel, Slectin, endothelial cell, ligand
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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