Recombinant Anti-GITR Antibody [DM78] - BSA and Azide free (A317382)

Name: Anti-GITR Antibody [DM78] - BSA and Azide free
Brand: Antibodies.com
SKU: A317382
Price: 665.0 USD
Availability: InStock

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$665

Recombinant rabbit monoclonal [DM78] antibody to GITR for ELISA and Flow Cytometry.

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Freight Charges

$40 to United States

Lead Time

7-8 Business Days

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Recombinant Antibody

Specifications

Name

Anti-GITR Antibody [DM78] - BSA and Azide free

Description

Recombinant rabbit monoclonal [DM78] antibody to GITR.

Applications

ELISA, Flow Cytometry

Dilutions

ELISA: 1:5,000-10,000, Flow Cytometry: 1:100

Reactivity

Human

Host

Rabbit

Clonality

Monoclonal

Clone ID

DM78

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity chromatography.

Concentration

Reconstitution dependent.

Product Form

Lyophilized

Reconstitution

Reconstitute with distilled sterile water.

Formulation

Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.

Storage

Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.

General Notes

Prior to reconstitution, centrifuge the vial at 5,000g for 3-5 minutes at room temperature. Reconstitute with appropriate volume of distilled sterile water to bring product to 1mg/ml concentration. After addition of distilled sterile water, mix by gentle tapping. Note: It is not recommended to vortex or vigorously pipette the sample.

Synonyms

Activation inducible TNFR family receptor, Activation-inducible TNFR family receptor, AITR, CD357, GITR D, GITR-D, GITRD, Glucocorticoid induced TNFR related protein, glucocorticoid-induced tnf receptor ligand, glucocorticoid-induced tnf receptors ligand, Glucocorticoid-induced TNFR-related protein, TNF receptor superfamily activation inducible protein, TNFRSF 18, TNFRSF18, TNR18_HUMAN, Tumor necrosis factor receptor superfamily member 18, Tumor necrosis factor receptor superfamily member 18 precursor

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Anti-GITR Antibody [DM78] - BSA and Azide free (A317382)

ELISA plates were pre-coated with Recombinant Human GITR Protein (Fc Chimera 6xHis Tag) (A318390) at 2 µg/ml (100 µl/well) which can bind Anti-GITR Antibody [DM78] - BSA and Azide free (A317382) in a linear range of 1-100 ng/ml.

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Figure 2: Flow Cytometry - Anti-GITR Antibody [DM78] - BSA and Azide free (A317382)

Expi 293 cell line was transfected with irrelevant protein (A) and human GITR (B) were surface stained with Anti-GITR Antibody [DM78] - BSA and Azide free (A317382) at 1µg/ml followed by Anti-Rabbit IgG Antibody (Alexa 488).

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Figure 3: Flow Cytometry - Anti-GITR Antibody [DM78] - BSA and Azide free (A317382)

Flow cytometry data of serially titrated Anti-GITR Antibody [DM78] - BSA and Azide free (A317382) on PC3 cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.

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Figure 4: Flow Cytometry - Anti-GITR Antibody [DM78] - BSA and Azide free (A317382)

Affinity ranking of different Rabbit Anti-GITR Monoclonal Antibody clones by titration of different concentrations onto PC3 cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.

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