Anti-GIT1 Antibody [S39B-8] (A304764)

Name: Anti-GIT1 Antibody [S39B-8]
Brand: Antibodies.com
SKU: A304764
Price: 500.0 USD
Availability: InStock

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$500

Mouse monoclonal [S39B-8] antibody to GIT1 for WB, ICC/IF and IP.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-GIT1 Antibody [S39B-8]

Description

Mouse monoclonal [S39B-8] antibody to GIT1.

Applications

WB, ICC/IF, IP

Dilutions

WB: 1:1,000

Reactivity

Human, Mouse, Rat

Cross Reactivity

This antibody does not cross-react with GIT2.

Immunogen

Fusion protein amino acids 375-770 (C-terminus) of rat GIT1.

Host

Mouse

Clonality

Monoclonal

Clone ID

S39B-8

Isotype

IgG1

Conjugate

Unconjugated

Purification

Protein G purification.

Concentration

1 mg/ml

Molecular Weight

~90 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

ARF GAP GIT1, ARF GTPase activating protein GIT1, ARF GTPase-activating protein GIT1, CAT 1, CAT-1, CaT1, Cool associated and tyrosine phosphorylated protein 1, Cool-associated and tyrosine-phosphorylated protein 1, G protein coupled receptor kinase interacting ArfGAP 1, G protein coupled receptor kinase interactor 1, G protein-coupled receptor kinase-interactor 1, GIT1_HUMAN, GRK interacting protein 1, GRK-interacting protein 1

Isotype Controls

Suitable Secondaries

See all Anti-Mouse IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Immunofluorescence - Anti-GIT1 Antibody [S39B-8] (A304764)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cells (SH-SY5Y), fixed in 4% PFA for 15 min, using Anti-GIT1 Antibody [S39B-8] (A304764), at 1:50 for overnight at 4°C with slow rocking. The secondary antibody used was AlexaFluor 488 at 1:1,000 for 1 hour at room temperature. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 minutes at room temperature.(A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GIT1 Antibody (D) Composite.

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Figure 2: Western Blot - Anti-GIT1 Antibody [S39B-8] (A304764)

Western blot analysis of rat brain membrane lysate showing detection of GIT1 protein using Anti-GIT1 Antibody [S39B-8] (A304764) at 1:1,000.

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Figure 3: Immunofluorescence - Anti-GIT1 Antibody [S39B-8] (A304764)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cell line (SK-N-BE, fixed in 4% formaldehyde for 15 min at room temperature, using Anti-GIT1 Antibody [S39B-8] (A304764), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Mouse ATTO 488 at 1:100 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5,000 for 60 minutes room temperature, 5 minutes room temperature. Localization: Cytoplasm. Magnification: 60X.(A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) GIT1 Antibody. (D) Composite.

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