Recombinant mouse monoclonal [rGFAP/8685] antibody to GFAP.
Specificity
This MAb recognizes a protein of ~50kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.
Applications
IHC-P
Positive Control
T98G cells. Brain or Astrocytoma
Dilutions
IHC: 0.25-2 µg/ml
Reactivity
Human, Mouse, Rat, Bovine, Porcine, Rabbit, Chicken
Immunogen
Recombinant fragment (around Amino Acids 150-350) of human GFAP protein. The exact sequence is proprietary.
Host
Mouse
Clonality
Monoclonal
Clone ID
rGFAP/8685
Isotype
IgG1
Light Chains
kappa
Conjugate
Unconjugated
Purification
Protein A/G chromatography.
Concentration
200 µg/ml
Molecular Weight
~50 kDa
Product Form
Liquid
Formulation
Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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