Anti-GFAP Antibody [3E10] (A270551)

Immunofluorescent analysis of rat hippocampus section stained with Anti-GFAP Antibody [3E10] (A270551), at a dilution of 1:500, in red, and co-stained with Anti-MAP2 Antibody (A85363), at a dilution of 1:5,000, in green. Nuclear DNA is visualised in blue using Hoechst staining. Following transcardial perfusion with 4% paraformaldehyde, the brain was post-fixed for 24 hours, cut to 45 µm, and free-floating sections were stained using the above antibodies. Anti-GFAP Antibody [3E10] (A270551) stains the network of astroglial cells while Anti-MAP2 Antibody (A85363) labels perikarya and dendrites of neurons.

Western blot analysis of equal amount of total proteins from different cell and tissue lysates using Anti-GFAP Antibody [3E10] (A270551), at a dilution of 1:1,000, in green. The lanes contain: [Lane 1] protein standards (red), [Lane 2] primary rat cortical neuron-glial cells, [Lane 3] rat brain, [Lane 4] mouse brain, [Lane 5] cow cortex, [Lane 6] cow cerebellum, and [Lane 7] pig hippocampus. Bands around 50 kDa correspond to GFAP protein and its alternative transcripts and fragments. Anti-GFAP Antibody [3E10] (A270551) has stronger reactivity with cow GFAP and pig GFAP compared to rodent GFAP; and also reacts strongly with human native and recombinant GFAP (not shown). The same blot was probed with Anti-UCHL1 Antibody (A85349), a neuron cell marker, at a dilution of 1:2,000, in red. Anti-UCHL1 Antibody (A85349) reveals a 25 kDa band corresponding to UCHL1 protein detected in all preparations, acting as a positive loading control.

Immunofluorescent analysis of mouse hippocampus section stained with Anti-GFAP Antibody [3E10] (A270551) at a dilution of 1:500 (green) and costained with Anti-NF-L Antibody (A85286) at a dilution of 1:2,000 (red). Nuclei were stained with Hoechst (blue). Following transcardial perfusion of mouse with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45µM, and free-floating sections were stained with the above antibodies. The GFAP antibody stains network of glial cells while the Anti-NF-L Antibody antibody labels axons and dendrites of neuronal cells.

Immunohistochemistry analysis of a formalin fixed paraffin embedded human brain cortex section with Anti-GFAP Antibody [3E10] (A270551) at a dilution of 1:1,000 detected with DAB (brown) using the Vector Labs ImmPRESS method and reagents with citra buffer retrieval. Counterstained with Hematoxylin (blue). In cortex, GFAP strongly labels astrocytes and glial cells. This antibody performs well in staining with 4% PFA or standard NBF fixed rat and human tissues. Anti-GFAP Antibody [3E10] (A270551) is the recommended and preferred clone for GFAP paraffin staining applications.