Anti-GFAP Antibody [3E10] (A270551)

Immunofluorescent analysis of rat hippocampus section stained using Anti-GFAP Antibody [3E10] (A270551), at a dilution of 1:500, in red. The tissue was co-stained using Anti-MAP2 Antibody (A85363), at a dilution of 1:5,000, in green. Nuclear DNA is visualised in blue using Hoechst staining. Following transcardial perfusion of the rat with 4% paraformaldehyde, the brain was post-fixed for 24 hours, cut to 45 µm, and free-floating sections were stained using the above antibodies. The Anti-GFAP Antibody [3E10] (A270551) stains the network of astroglial cells while the Anti-MAP2 Antibody (A85363) labels perikarya and dendrites of neurons.

Western blot analysis of equal amount of total proteins from different cell and tissue lysates using Anti-GFAP Antibody [3E10] (A270551), at a dilution of 1:1,000, in green. The lanes contain samples of: [1] Protein standards, in red, [2] primary rat cortical neuron-glial cells, [3] rat brain, [4] mouse brain, [5] cow cortex, [6] cow cerebellum, and [7] pig hippocampus. Bands around 50 kDa correspond to GFAP protein and its alternative transcripts and fragments. The Anti-GFAP Antibody [3E10] (A270551) has stronger reactivity with cow and pig GFAP as compared to rodent, and reacts strongly with human native and recombinant GFAP (not shown). The same blot was probed with Anti-UCHL1 Antibody (A85349), a neuron cell marker, at a dilution of 1:2,000, in red. The UCHL1 antibody reveals a 25 kDa band corresponding to UCHL1 protein detected in all preparations, acting as a positive loading control.