Anti-Geminin Antibody [GMNN/4033] (A277735)

Anti-Geminin Antibody [GMNN/4033]

Mouse monoclonal [GMNN/4033] antibody to Geminin.

Geminin is a nuclear protein that regulates the initiation of DNA replication during the cell cycle. DNA replication requires the coordinated association of Cdc6 and minichromosome maintenance (MCM) proteins with chromatin. Geminin blocks this assembly of the MCM into the prereplication complex and, in turn, prevents replication from occurring. Expression of Geminin fluctuates throughout the cell cycle with Geminin levels lowest at G1. Throughout S, G2 and M phases, Geminin levels are consistently elevated followed by a decrease during mitosis. The initiation of DNA replication is dependent on the degradation of Geminin during mitosis and the absence of Geminin throughout G1 phase. Geminin degradation is mediated by the anaphase-promoting complex (APC), which specifically targets B-type cyclins and other proteins containing a destruction box motif for degradation by ubiquitin-mediated proteolysis. While geminin expression is essential in maintaining chromosomal integrity, it is frequently overexpressed in cancers and evidence suggests that it plays a significant role in tumor proliferation and progression.

IHC-P: 1-2 µg/ml

Human

Recombinant fragment, around amino acids 71-202, of human Geminin protein. The exact sequence is proprietary.

Mouse

Monoclonal

GMNN/4033

IgG1

Unconjugated

Protein A/G chromatography.

200 µg/ml

35 kDa

Liquid

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

This monoclonal antibody is also available in a different formulation without BSA and Sodium Azide - Anti-Geminin Antibody [GMNN/4033] - BSA and Azide free (A278323).

DNA replication inhibitor, Gem, Geminin DNA replication inhibitor, GEMI_HUMAN, GMNN, OTTHUMP00000039393, RP3 369A17.3