Anti-Gelsolin Antibody (A82992)

Name: Anti-Gelsolin Antibody
Brand: Antibodies.com
SKU: A82992
Price: 480.0 USD
Availability: InStock

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$480

Goat polyclonal antibody to Gelsolin for ELISA, WB and Flow Cytometry.

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Delivery

Freight Charges

$40 to United States

Lead Time

3-5 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-Gelsolin Antibody

Description

Goat polyclonal antibody to Gelsolin.

Applications

ELISA, WB, Flow Cytometry

Dilutions

ELISA: 1:128,000, WB: 0.1-2µg/ml, Flow Cytometry: 10µg/ml

Reactivity

Human

Immunogen

Synthetic peptide corresponding to the internal region of human Gelsolin.

Sequence

C-PRLKDKKMDAHP

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Molecular Weight

90 kDa

Predicted MW

85.7kDa

Product Form

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

Actin depolymerizing factor, Actin-depolymerizing factor, ADF, AGEL, Brevin, DKFZp313L0718, GELS_HUMAN, Gsn

Isotype Controls

Goat IgG (A121671)

Suitable Secondaries

See all Anti-Goat IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (2)

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Figure 1: Western Blot - Anti-Gelsolin Antibody (A82992)

Gelsolin expression in Human Colorectal Cancer lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-Gelsolin Antibody (A82992) at 1µg/ml and detected by chemiluminescence.

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Figure 2: Flow Cytometry - Anti-Gelsolin Antibody (A82992)

Gelsolin expression in HeLa cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-Gelsolin Antibody (A82992) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.

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