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Anti-GDF15 Antibody (A16768)

Western Blot - Anti-GDF15 Antibody (A16768) - Antibodies.com Immunohistochemistry - Anti-GDF15 Antibody (A16768) - Antibodies.com Immunofluorescence - Anti-GDF15 Antibody (A16768) - Antibodies.com Western Blot - Anti-GDF15 Antibody (A16768) - Antibodies.com
This product is discontinued

Anti-GDF15 Antibody (A16768) has been discontinued and is no longer available.

View all GDF15 Antibodies.

  • Specifications

Name
Anti-GDF15 Antibody
Description
Rabbit polyclonal antibody to GDF15.
Applications
WB, IHC, ICC/IF, IP
Dilutions
WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 30-200 of human GDF15 (NP_004855.2).
Sequence
LSLAEASRASFPGPSELHSEDSRFRELRKRYEDLLTRLRANQSWEDSNTDLVPAPAVRILTPEVRLGSGGHLHLRISRAALPEGLPEASRLHRALFRLSPTASRSWDVTRPLRRQLSLARPQAPALHLRLSPPPSQSDQLLAESSSARPQLELHLRPQAARGRRRARARNG
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
35 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
GDF-15, Growth/differentiation factor 15, Macrophage inhibitory cytokine 1, MIC-1, MIC1, NAG-1, NRG-1, NSAID-activated gene 1 protein, NSAID-regulated gene 1 protein, PDF, PLAB, Placental bone morphogenetic protein, Placental TGF-beta, Prostate differentiation factor, PTGFB
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (4)

Western Blot - Anti-GDF15 Antibody (A16768) - Antibodies.comEnlarge Image

Western Blot - Anti-GDF15 Antibody (A16768)

Western blot analysis of extracts of various cell lines, using Anti-GDF15 Antibody (A16768) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry - Anti-GDF15 Antibody (A16768) - Antibodies.comEnlarge Image

Immunohistochemistry - Anti-GDF15 Antibody (A16768)

Immunohistochemistry analysis of paraffin-embedded human placenta using Anti-GDF15 Antibody (A16768) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunofluorescence - Anti-GDF15 Antibody (A16768) - Antibodies.comEnlarge Image

Immunofluorescence - Anti-GDF15 Antibody (A16768)

Immunofluorescence analysis of BALB-3T3 cells using Anti-GDF15 Antibody (A16768) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).
Western Blot - Anti-GDF15 Antibody (A16768) - Antibodies.comEnlarge Image

Western Blot - Anti-GDF15 Antibody (A16768)

Immunoprecipitation analysis of 200µg extracts of HT-1080 cells using 3µg of Anti-GDF15 Antibody (A16768). This Western blot was performed on the immunoprecipitate using Anti-GDF15 Antibody (A16768) at a dilution of 1:1000.

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Proteins predicted to interact with GDF15

Predicted protein interactions based upon String database. Revelancy score correlates with probability of interaction.
96% Relevancy Score
95.8% Relevancy Score
88.5% Relevancy Score
87.7% Relevancy Score
76.2% Relevancy Score
74.6% Relevancy Score
72.2% Relevancy Score
70.5% Relevancy Score
68.5% Relevancy Score