Anti-GDF15 Antibody (A16768)

Name: Anti-GDF15 Antibody
Brand: Antibodies.com
SKU: A16768
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to GDF15 for WB, IHC, ICC/IF and IP.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-GDF15 Antibody

Description

Rabbit polyclonal antibody to GDF15.

Applications

WB, IHC, ICC/IF, IP

Dilutions

WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 30-200 of human GDF15 (NP_004855.2).

Sequence

LSLAEASRASFPGPSELHSEDSRFRELRKRYEDLLTRLRANQSWEDSNTDLVPAPAVRILTPEVRLGSGGHLHLRISRAALPEGLPEASRLHRALFRLSPTASRSWDVTRPLRRQLSLARPQAPALHLRLSPPPSQSDQLLAESSSARPQLELHLRPQAARGRRRARARNG

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

35 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

GDF 15, GDF-15, GDF15_HUMAN, Growth differentiation factor 15, Growth/differentiation factor 15, Macrophage inhibitory cytokine 1, MIC 1, Mic-1, MIC1, NAG 1, NAG-1, NAG1, NRG 1, NRG-1, NRG1, NSAID, NSAID (nonsteroidal anti inflammatory drug) activated protein 1, NSAID regulated protein 1, NSAID-activated gene 1 protein, NSAID-regulated gene 1 protein, PDF, PLAB, Placental bone morphogenetic protein, Placental bone morphogenic protein, Placental TGF beta, Placental TGF-beta, Prostate differentiation factor, PTGF beta, PTGFB

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (4)

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Figure 1: Western Blot - Anti-GDF15 Antibody (A16768)

Western blot analysis of extracts of various cell lines, using Anti-GDF15 Antibody (A16768) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Figure 2: Immunohistochemistry - Anti-GDF15 Antibody (A16768)

Immunohistochemistry analysis of paraffin-embedded human placenta using Anti-GDF15 Antibody (A16768) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 3: Immunofluorescence - Anti-GDF15 Antibody (A16768)

Immunofluorescence analysis of BALB-3T3 cells using Anti-GDF15 Antibody (A16768) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

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Figure 4: Western Blot - Anti-GDF15 Antibody (A16768)

Immunoprecipitation analysis of 200µg extracts of HT-1080 cells using 3µg of Anti-GDF15 Antibody (A16768). This Western blot was performed on the immunoprecipitate using Anti-GDF15 Antibody (A16768) at a dilution of 1:1000.

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