Figure 1: Western Blot - Anti-GARS Antibody [ARC0514] (A308454)
Western blot analysis of Jurkat, using Anti-GARS Antibody [ARC0514] (A308454) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
Figure 2: Western Blot - Anti-GARS Antibody [ARC0514] (A308454)
Western blot analysis of extracts from wild type(WT) and GARS knockout (KO) 293T cells, using Anti-GARS Antibody [ARC0514] (A308454) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
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