Mouse monoclonal [TU-30] antibody to gamma Tubulin.
Specificity
This antibody recognizes the C terminus of human gamma Tubulin, a 48 kDa structural constituent of cytoskeleton and microtubule organizing center (MTOC). The epitope is located in the amino acid sequence TRPDYI (a.a. 439-444 in human), which is present on human gamma Tubulin 1 but not on human gamma Tubulin 2.
Applications
ICC, WB, Flow Cytometry (Intracellular)
Dilutions
IHC: 1-2 µg/ml. Staining technique: (a) Fix cells for 10 min in methanol at -20°C and for 6 min in acetone at -20°C; (b) Fix cells directly in methanol for 10 min at -20°C or in acetone for 10 min at -20°C. Positive Control: P-19 murine embryonal carcinoma cell line, 3T3 murine fibroblasts. The antibody TU-30 stains only fixed cells, WB: Recommended dilution 1-2 µg/ml; Reducing conditions.
Reactivity
Protozoa, Chicken, Bovine, Rat, Mouse, Porcine, Human, Plants
Immunogen
C-terminal peptide of gamma-tubulin counjugated to KLH.
Host
Mouse
Clonality
Monoclonal
Clone ID
TU-30
Isotype
IgG1
Conjugate
Unconjugated
Purification
Protein A chromatography.
Concentration
1 mg/ml
Predicted MW
48 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Western Blot - Anti-gamma Tubulin Antibody [TU-30] (A86254)
Differential reactivity of monoclonal antibodies to ?-tubulin with human ?-tubulin isotypes. (A) Immunoblots of total cell lysates from SH-SY5Y cells, expressing TagRFP-tagged human ?-tubulin 1 (?-Tb1) or ?-tubulin 2 (?-Tb2), probed with Abs to ?-tubulin (TU-30, TU-32), TagRFP (RFP) and GAPDH. In control samples, only secondary anti-mouse Ab was applied. (B) Immunoblots of immobilized GST-tagged human C-terminal regions (a.a. 362-451) of ?-Tb1 or ?-Tb2 probed with Abs to ?-tubulin (TU-30, TU-32) and GST. In control samples, only secondary anti-mouse Ab was applied.
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