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Anti-GABARAPL2 Antibody (A305138)

Western Blot - Anti-GABARAPL2 Antibody (A305138) - Antibodies.com Immunocytochemistry/Immunofluorescence - Anti-GABARAPL2 Antibody (A305138) - Antibodies.com
$500
Product Datasheet

Rabbit polyclonal antibody to GABARAPL2 for WB and ICC/IF.

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$40 to United States
Lead Time
5-8 Business Days

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Name
Anti-GABARAPL2 Antibody
Description
Rabbit polyclonal antibody to GABARAPL2.
Applications
WB, ICC/IF
Dilutions
WB: 1:1,000, ICC/IF: 1:100
Reactivity
Human, Rat
Immunogen
Synthetic peptide from the C-terminal of Human GABARAPL2.
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Peptide affinity purification.
Concentration
1 mg/ml
Molecular Weight
~14 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 50% Glycerol and 0.09% Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
ATG8, ATG8C, FLC3A, GABA(A) receptor-associated protein-like 2, Gamma-aminobutyric acid receptor-associated protein-like 2, Ganglioside expression factor 2, GATE-16, GATE16, GBRL2_HUMAN, GEF-2, GEF2, General protein transport factor p16, Golgi-associated ATPase enhancer of 16 kDa, MAP1 light chain 3-related protein
Isotype Controls
Suitable Secondaries
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - Anti-GABARAPL2 Antibody (A305138) - Antibodies.comEnlarge Image
Figure 1: Western Blot - Anti-GABARAPL2 Antibody (A305138)
Western blot analysis of rat liver cell lysates showing detection of ~14 kDa GABARAPL2 protein using Anti-GABARAPL2 Antibody (A305138) at 1:1,000 for 60 minutes at room temperature. Lane 1: Molecular Weight Ladder (MW). Lane 2: rat liver cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 minutes at room temperature. Color Development: ECL solution for 6 min in room temperature. Predicted/Observed Size: ~14 kDa.
Immunocytochemistry/Immunofluorescence - Anti-GABARAPL2 Antibody (A305138) - Antibodies.comEnlarge Image
Figure 2: Immunofluorescence - Anti-GABARAPL2 Antibody (A305138)
Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cell line (SK-N-BE, fixed in 4% formaldehyde for 15 min at room temperature, using Anti-GABARAPL2 Antibody (A305138), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Rabbit ATTO 488 at 1:100 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5,000 for 60 minutes at room temperature, 5 minutes at room temperature. Localization: Cytoplasmic Vesicle, Autophagosome. Magnification: 60X.(A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) GABARAPL2 Antibody. (D) Composite.
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