Anti-GABARAP Antibody (A304935)

Name: Anti-GABARAP Antibody
Brand: Antibodies.com
SKU: A304935
Price: 500.0 USD
Availability: InStock

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$500

Rabbit polyclonal antibody to GABARAP for WB and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-GABARAP Antibody

Description

Rabbit polyclonal antibody to GABARAP.

Specificity

Other observed bands at ~40 kDa. which is a complex, and ~26 kDa which is a dimer.

Applications

WB, ICC/IF

Dilutions

WB: 1:1,000, ICC/IF: 1:100

Reactivity

Human, Mouse

Immunogen

Synthetic peptide from the C-terminal of human GABARAP.

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Peptide affinity purification.

Concentration

1 mg/ml

Molecular Weight

~14-16 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline with 50% Glycerol and 0.09% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

ATG8A, FLC 3B, FLC3B, FLJ25768, GABA type A receptor associated protein, GABA(A) receptor associated protein, GABA(A) receptor-associated protein, GABARAP a, Gamma aminobutyric acid receptor associated protein, Gamma-aminobutyric acid receptor-associated protein, GBRAP_HUMAN, MGC120154, MGC120155, MM 46, MM46

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (3)

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Figure 1: Western Blot - Anti-GABARAP Antibody (A304935)

Western blot analysis of mouse kidney showing detection of ~13.9kDa GABARAP protein using Anti-GABARAP Antibody (A304935) at 1:1,000 for 1 hour at room temperature. Lane 1: MW Ladder. Lane 2: mouse kidney (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at room temperature. The secondary antibody used was Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at room temperature. Color Development: TMB solution for 12 min at room temperature. Predicted/Observed Size: ~13.9kDa. Other Band(s): ~40kDa (potential trimer).

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Figure 2: Western Blot - Anti-GABARAP Antibody (A304935)

Western blot analysis of human Embryonic kidney epithelial cell line (HEK293T) lysate showing detection of ~13.9kDa GABARAP protein using Anti-GABARAP Antibody (A304935) at 1:1,000 for 1 hour at room temperature. Lane 1: MW Ladder. Lane 2: human 293T (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at room temperature. The secondary antibody used was Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at room temperature. Color Development: TMB solution for 12 min at room temperature. Predicted/Observed Size: ~13.9kDa. Other Band(s): ~40kDa (potential trimer).

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Figure 3: Immunofluorescence - Anti-GABARAP Antibody (A304935)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cell line (SK-N-BE, fixed in 4% formaldehyde for 15 min at room temperature, using Anti-GABARAP Antibody (A304935), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Rabbit ATTO 488 at 1:100 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5,000 for 60min room temperature, 5min room temperature. Localization: Endomembrane System, Cytoplasm, Cytoskeleton, Golgi Apparatus Membrane, Cytoplasmic Vesicle. Magnification: 60X.(A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) GABARAP Antibody (D) Composite.

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