Anti-Fyn Antibody (A309065)

Name: Anti-Fyn Antibody
Brand: Antibodies.com
SKU: A309065
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to Fyn for WB, IHC and ICC/IF.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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+1 (314) 370-6046

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Specifications

Name

Anti-Fyn Antibody

Description

Rabbit polyclonal antibody to Fyn.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:100-1:500, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 200-300 of human Fyn (NP_002028.1).

Sequence

VKHYKIRKLDNGGYYITTRAQFETLQQLVQHYSERAAGLCCRLVVPCHKGMPRLTDLSVKTKDVWEIPRESLQLIKRLGNGQFGEVWMGTWNGNTKVAIKT

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

61 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

C syn protooncogene, FYN oncogene related to SRC FGR YES, FYN_HUMAN, OKT3 induced calcium influx regulator, P59 FYN, p59-Fyn, Protein tyrosine kinase fyn, Proto oncogene tyrosine protein kinase fyn, Proto-oncogene c-Fyn, Proto-oncogene Syn, Protooncogene Syn, SLK, Src like kinase, Src yes related novel gene, Src-like kinase, Src/yes related novel, SYN, Tyrosine kinase p59fyn T, Tyrosine kinase p59fyn(T), Tyrosine-protein kinase Fyn

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (5)

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Figure 1: Western Blot - Anti-Fyn Antibody (A309065)

Western blot analysis of extracts of various cell lines, using Anti-Fyn Antibody (A309065) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.

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Figure 2: Western Blot - Anti-Fyn Antibody (A309065)

Western blot analysis of extracts of Rat brain, using Anti-Fyn Antibody (A309065) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 180s.

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Figure 3: Immunohistochemistry - Anti-Fyn Antibody (A309065)

Immunohistochemistry analysis of paraffin-embedded human placenta using Anti-Fyn Antibody (A309065) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Figure 4: Immunofluorescence - Anti-Fyn Antibody (A309065)

Immunofluorescence analysis of Jurkat cells using Anti-Fyn Antibody (A309065) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).