Anti-FTO Antibody (A8927)

Name: Anti-FTO Antibody
Brand: Antibodies.com
SKU: A8927
Price: 335.0 USD
Availability: InStock

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$335

Rabbit polyclonal antibody to FTO for WB and IP.

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Delivery

Freight Charges

$40 to United States

Lead Time

5-8 Business Days

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Telephone

+1 (314) 370-6046

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Specifications

Name

Anti-FTO Antibody

Description

Rabbit polyclonal antibody to FTO.

Applications

WB, IP

Dilutions

WB: 1:500-1:1,000, IP: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human FTO (NP_001073901.1).

Sequence

MKRTPTAEEREREAKKLRLLEELEDTWLPYLTPKDDEFYQQWQLKYPKLILREASSVSEELHKEVQEAFLTLHKHGCLFRDLVRIQGKDLLTPVSRILIGNPGCTYKYLNTRLFTVPWPVKGSNIKHTEAEIAAACETFLKLNDYLQIETIQALEELAAKEKANEDAVPLCMSADFPRVGMGSSYNGQDEVDIKSRAAYNVTLLNFMDPQKMPYLKEEPYFGMGKMAVSWHHDENLVDRS

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

60 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

AlkB homolog 9, ALKBH9, Alpha-ketoglutarate-dependent dioxygenase FTO, AW743446, Fat mass and obesity-associated protein, FATSO, MOUSE, HOMOLOG OF, FTO_HUMAN, GDFD, KIAA1752, mKIAA1752, Protein fatso

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation Data (2)

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Figure 1: Western Blot - Anti-FTO Antibody (A8927)

Western blot analysis of extracts of various cell lines, using Anti-FTO Antibody (A8927) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Figure 2: Western Blot - Anti-FTO Antibody (A8927)

Immunoprecipitation analysis of 300µg extracts of SH-SY5Y cells using 3µg of Anti-FTO Antibody (A8927). This Western blot was performed on the immunoprecipitate using Anti-FTO Antibody (A8927) at a dilution of 1:1000.

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