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FRK Cell Based ELISA Kit (A103341)

Western Blot - FRK Cell Based ELISA Kit (CB5891) - Antibodies.com Protocol Diagram - FRK Cell Based ELISA Kit (CB5891) - Antibodies.com Protocol Summary - FRK Cell Based ELISA Kit (CB5891) - Antibodies.com Western Blot - Control Antibody - FRK Cell Based ELISA Kit (CB5891) - Antibodies.com
$550
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$40 to United States
Lead Time
5-8 Business Days

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Name
FRK Cell Based ELISA Kit
Assay Type
Cell-based (qualitative)
Principle of Assay
This FRK Cell Based ELISA Kit allows for the detection of FRK and the effects that certain stimulation conditions have on FRK expression in different cell lines. Qualitative determination of FRK concentration is achieved by an indirect ELISA format. In essence, the FRK is captured by Anti-FRK Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this FRK Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.
Platform
Microplate
Detection Type
Colorimetric
Sample Type
Adherent cells and suspension cells.
Product Range
> 5000 Cells
Assay Time
4 hours 30 minutes
Reactivity
Human, Mouse, Rat
Recovery
Storage
Store at + 4°C. Stable for 6 months.
Synonyms
Beta cell Src homology tyrosine kinase, BSK, BSK/IYK, C85044, Frk fyn related kinase, FRK_HUMAN, FYN related kinase, FYN-related kinase, GASK, Gastrointestinal associated kinase, Gastrointestinal associated tyrosine kinase, GTK, GTK, mouse, homolog of, Intestine tyrosine kinase, Nuclear tyrosine protein kinase RAK, Protein-tyrosine kinase 5, PTK5, PTK5 protein tyrosine kinase 5, RAK, Tyrosine protein kinase FRK, Tyrosine-protein kinase FRK
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
Western Blot - FRK Cell Based ELISA Kit (CB5891) - Antibodies.comEnlarge Image
Figure 1: Western Blot - FRK Cell Based ELISA Kit (A103341)
Western blot analysis of extracts from Jurkat cells, using Anti-FRK Antibody.
Protocol Diagram - FRK Cell Based ELISA Kit (CB5891) - Antibodies.comEnlarge Image
Figure 2: FRK Cell Based ELISA Kit (A103341)
A diagram showing the protocol for FRK Cell Based ELISA Kit.
Protocol Summary - FRK Cell Based ELISA Kit (CB5891) - Antibodies.comEnlarge Image
Figure 3: FRK Cell Based ELISA Kit (A103341)
A summary of the protocol for FRK Cell Based ELISA Kit.
Western Blot - Control Antibody - FRK Cell Based ELISA Kit (CB5891) - Antibodies.comEnlarge Image
Figure 4: Western Blot - FRK Cell Based ELISA Kit (A103341)
The mouse monoclonal antibody to GAPDH is used as a positive control inFRK Cell Based ELISA Kit. Anti-GAPDH Antibody was tested for specificity by western blot with tissue lysates from human, mouse, and rat.
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