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Anti-XPA Anticorps [5F12] (A355)

Ce produit est identique à Abcam (ab65963). Découvrez plus pour moins cher.

Western blot of endogenous XPA protein. Hela cell whole extract (20µg). Anti-XPA Antibody was used at 1:2,000 dilution. Goat Anti-Mouse IgG Antibody (HRP) was used as a secondary at 1:20,000 dilution. Western blot of XPA. Lane 1. Extract of Hela cells (XPA wild type). Lane 2. Extract of XP12ROSV cells (XPA deficient). The primary and the secondary antibodies were used at 1:2,000 and 1:20,000 dilutions. Immunofluorescence staining of human fibroblast cells (GM0637) using Anti-XPA Antibody. The cells were non-irradiated (left) or irradiated with UV at 20 J/m2 and fixed after 30 min with paraformaldehyde. Anti-XPA Antibody was used at 1:100 dilution and Goat Anti-Mouse IgG (Alexa 448) was used as a secondary at 1:5,000 dilution.
Anticorps Validé par Knockout
$535

Anticorps monoclonal [5F12] de souris contre XPA pour WB, IF, ELISA et Inhibition Assay.

Qualité 100 % Garantie
Garantie de Correspond­ance des Prix

Frais de livraison
$40
Date de livraison
Livraison sous 4-6 jours ouvrables.

Téléphone
+1 (314) 370-6046
Lun - Ven, 8h - 16h AST
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orders@antibodies.com

Nom du produit
Anti-XPA Antibody [5F12]
Description du produit
Mouse monoclonal (5F12) antibody to XPA.
Applications
WB, IF, ELISA, Inhibition Assay
Recommander des dilutions
WB: 1:1,000-1:10,000, IF: 1:100-1:1,000
Reactivité
Human
Immunogène
Recombinant full length human XPA protein.
Épitope
Amino acids 30-47.
Hôte
Mouse
Clonalité
Monoclonal
Clone
5F12
Isotype
IgG2b
Conjuguer

Unconjugated

Purification
Ion exchange chromatography.
Concentration
1 mg/ml
Forme du produit
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.2, with 50% Glycerol (filter sterilised, without Sodium Azide, and carrier free).
Stockage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonymes
DNA repair protein complementing XP A cells, DNA repair protein complementing XP-A cells, DNA repair protein complementing XPA cells, Excision repair controlling, Xeroderma pigmentosum 1, Xeroderma pigmentosum complementation group A, Xeroderma pigmentosum group A complementing protein, Xeroderma pigmentosum group A-complementing protein, XP 1, XP1, Xpac, XPA_HUMAN
Contrôles isotype
Anticorps secondaires appropriés
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Western blot of endogenous XPA protein. Hela cell whole extract (20µg). Anti-XPA Antibody was used at 1:2,000 dilution. Goat Anti-Mouse IgG Antibody (HRP) was used as a secondary at 1:20,000 dilution.Cliquez pour ouvrir le point de vue élargi
Figure 1: Anti-XPA Antibody [5F12] (70-031)
Anti-XPA Antibody
Western blot of XPA. Lane 1. Extract of Hela cells (XPA wild type). Lane 2. Extract of XP12ROSV cells (XPA deficient). The primary and the secondary antibodies were used at 1:2,000 and 1:20,000 dilutions.Cliquez pour ouvrir le point de vue élargi
Figure 2: Anti-XPA Antibody [5F12] (70-031)
Anti-XPA Antibody
Immunofluorescence staining of human fibroblast cells (GM0637) using Anti-XPA Antibody. The cells were non-irradiated (left) or irradiated with UV at 20 J/m2 and fixed after 30 min with paraformaldehyde. Anti-XPA Antibody was used at 1:100 dilution and Goat Anti-Mouse IgG (Alexa 448) was used as a secondary at 1:5,000 dilution.Cliquez pour ouvrir le point de vue élargi
Figure 3: Immunofluorescence - Anti-XPA Antibody [5F12] (70-031)
Anti-XPA Antibody

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