Anti-xCT Anticorps (A84265)

Name: Anti-xCT Antibody
Brand: Antibodies.com
SKU: A84265
Price: 480.0 USD
Availability: InStock

Ce produit est identique à Abcam (ab111822). Découvrez plus pour moins cher.

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$480

Anticorps polyclonal de chèvre contre xCT pour ELISA et IF.

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 3-5 jours ouvrables.

Nous ContacterContact

Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanties

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Spécifications

Nom du produit

Anti-xCT Antibody

Description du produit

Goat polyclonal antibody to xCT.

Applications

ELISA, IF

Recommander des dilutions

ELISA: 1:32,000, IF: 10µg/ml

Reactivité

Human

Immunogène

Synthetic peptide corresponding to the internal region of human xCT.

Séquence

C-KGQTQNFKDAFSGRD

Hôte

Goat

Clonalité

Polyclonal

Isotype

IgG

Conjuguer

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Forme du produit

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonymes

Amino acid transport system xc xCT, Amino acid transport system xc-, Calcium channel blocker resistance protein CCBR1, CCBR1, Cysteine/glutamate transporter, Cystine/glutamate transporter, OTTHUMP00000164578, SLC7A11, solute carrier family 7, Solute carrier family 7 (anionic amino acid transporter light chain, xc- system), member 11, Solute carrier family 7 member 11, Solute carrier family 7, (cationic amino acid transporter, y+ system) member 11, SYSTEM Xc(-) TRANSPORTER-RELATED PROTEIN, XCT_HUMAN

Contrôles isotype

Goat IgG (A121671)

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (7)

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Figure 1: Immunofluorescence - Anti-xCT Antibody (A84265)

xCT expression in HepG2 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-xCT Antibody (A84265) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Vesicle staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Figure 2: Immunofluorescence - Anti-xCT Antibody (A84265)

xCT expression in A549 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-xCT Antibody (A84265) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. ER/vesicle staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Figure 3: Western Blot - Anti-xCT Antibody (A84265)

xCT expression in Human Smooth Muscle (A) and Tonsil (B) lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-xCT Antibody (A84265) at 0.1µg/ml and detected by chemiluminescence.

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Figure 4: Western Blot - Anti-xCT Antibody (A84265)

xCT expression in A549 (A) and U2OS (B) cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-xCT Antibody (A84265) at 0.3µg/ml and detected by chemiluminescence.