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Anti-Wilms Tumor Protéine Anticorps [ARC2610] (A308215)

Nom du produit
Anti-Wilms Tumor Protein Antibody [ARC2610]
Description du produit
Rabbit monoclonal [ARC2610] antibody to Wilms Tumor Protein.
Applications
WB, IHC
Recommander des dilutions
WB: 1:500-1:1,000, IHC: 1:50-1:200
Reactivité
Human, Mouse, Rat
Immunogène
Recombinant fusion protein containing a sequence corresponding to amino acids 60-250 of human WT1 (P19544).
Séquence
PPPPPPPPPHSFIKQEPSWGGAEPHEEQCLSAFTVHFSGQFTGTAGACRYGPFGPPPPSQASSGQARMFPNAPYLPSCLESQPAIRNQGYSTVTFDGTPSYGHTPSHHAAQFPNHSFKHEDPMGQQGSLGEQQYSVPPPVYGCHTPTDSCTGSQALLLRTPYSSDNLYQMTSQLECMTWNQMNLGATLKGV
Hôte
Rabbit
Clonalité
Monoclonal
Clone
ARC2610
Isotype
IgG
Conjuguer

Unconjugated

Purification
Affinity purification.
Masse moléculaire
51 - 60 kDa
Forme du produit
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.02% Sodium Azide.
Stockage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonymes
AWT1, FWT1, GUD, NPHS4, WAGR, Wilms Tumor, Wilms tumor 1, Wilms' tumor gene, Wilms' tumor protein, WIT 2, WIT2, WT, WT 1, WT1, WT1_HUMAN, WT33
Contrôles isotype
Anticorps secondaires appropriés
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Western Blot - Anti-Wilms Tumor Protein Antibody [ARC2610] (A308215) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 1: Western Blot - Anti-Wilms Tumor Protein Antibody [ARC2610] (A308215)
Western blot analysis of extracts of various cell lines, using Anti-Wilms Tumor Protein Antibody [ARC2610] (A308215) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.
Western Blot - Anti-Wilms Tumor Protein Antibody [ARC2610] (A308215) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 2: Western Blot - Anti-Wilms Tumor Protein Antibody [ARC2610] (A308215)
Western blot analysis of extracts of Rat testis, using Anti-Wilms Tumor Protein Antibody [ARC2610] (A308215) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
Western Blot - Anti-Wilms Tumor Protein Antibody [ARC2610] (A308215) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 3: Western Blot - Anti-Wilms Tumor Protein Antibody [ARC2610] (A308215)
Western blot analysis of extracts of Mouse testis, using Anti-Wilms Tumor Protein Antibody [ARC2610] (A308215) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry - Anti-Wilms Tumor Protein Antibody [ARC2610] (A308215) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 4: Immunohistochemistry - Anti-Wilms Tumor Protein Antibody [ARC2610] (A308215)
Immunohistochemistry analysis of paraffin-embedded mouse kidney using Anti-Wilms Tumor Protein Antibody [ARC2610] (A308215) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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