This antibody recognises a subset of WC1+ T-cells expressing the WC1.1 isoform. The bovine WC1 cell surface antigen is expressed by a population of gamma/delta T-cells that lack CD2, CD4 and CD8, but express CD3. WC1 expression appears to be heterogeneous and antibodies to this cluster show differing reaction patterns. recognises the swine homolog of WC1, which is a 180 kDa molecule. In pigs, the 180 kDa molecule is expressed by a gamma/delta TCR positive T-cell population that also lack CD2, CD4 and CD8.

Applications

IP, Flow Cytometry, IHC-Fr

Recommander des dilutions

Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl.

Reactivité

Bovine, Pig, Sheep

Immunogène

Con A stimulated bovine lymphocytes.

Hôte

Mouse

Clonalité

Monoclonal

Clone

CC101

Isotype

IgG2a

Conjuguer

Unconjugated

Purification

Protein A affinity chromatography of tissue culture supernatant.

Concentration

1 mg/ml

Forme du produit

Liquid

Formulation

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Stockage

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Notes générales

Mouse anti Bovine WC1 antibody, clone CC101, recognizes a subset of WC1+ T-cells expressing the WC1.1 isoform (MacHugh et al. 1993).The bovine WC1 cell surface antigen is expressed by a population of gamma/delta T-cells that lack CD2, CD4 and CD8, but express CD3. WC1 expression appears to be heterogeneous and antibodies to this cluster show differing reaction patterns (Crocker et al. 1993). Mouse anti bovine WC1, clone CC101, immunoprecipitates a 215 kDa molecule from bovine cells and also recognizes the swine homolog of WC1, which is a 180 kDa molecule. In pigs, the 180 kDa molecule is expressed by a gamma/delta TCR positive T-cell population that also lack CD2, CD4 and CD8 (Carr et al. 1994).

Synonymes

ATP7b, ATP7B_HUMAN, ATPase, Cu(2+) transporting, beta polypeptide, ATPase, Cu++ transporting, beta polypeptide, Copper pump 2, Copper transporting ATPase 2, PWD, Toxic milk, tx, WD, Wilson disease associated protein, Wilson disease-associated protein, WND, WND/140 kDa

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.