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WAVE1 Cell Based Kit Elisa (A103125)

Nom du produit
WAVE1 Cell Based ELISA Kit
Type de test
Cell-based (qualitative)
Principe du test
This WAVE1 Cell Based ELISA Kit allows for the detection of WAVE1 and the effects that certain stimulation conditions have on WAVE1 expression in different cell lines. Qualitative determination of WAVE1 concentration is achieved by an indirect ELISA format. In essence, the WAVE1 is captured by Anti-WAVE1 Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this WAVE1 Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.
Plate-forme
Microplate
Méthode de détection
Colorimetric
Type d'échantillon
Adherent cells and suspension cells.
Gamme de detection
> 5000 Cells
Durée du test
4 hours 30 minutes
Reactivité
Human, Mouse, Rat
Precision
Intra-assay CV: < 8%, Inter-assay CV: < 10%
Stockage
Store at + 4°C. Stable for 6 months.
Synonymes
FLJ31482, homology of dictyostelium scar 1, KIAA0269, Protein WAVE-1, Protein WAVE1, scar, Dictyostelium, homology of, 1, SCAR1, Similar to a plant extensin like protein, Verprolin homology domain containing protein 1, Verprolin homology domain-containing protein 1, WAS protein family, member 1, Wasf1, WASF1_HUMAN, WASL, WASP family 1, WASP family member 1, WASP family protein member 1, WASP family, verprolin homology domain-containing protein, WASP family, verprolin homology domain-containing protein 1, WAVE, WAVE 1, Wiskott Aldrich syndrome protein family member 1, Wiskott-Aldrich syndrome protein family member 1
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Western Blot - WAVE1 Cell Based ELISA Kit (CB5728) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 1: Western Blot - WAVE1 Cell Based ELISA Kit (CB5728)
Western blot analysis of extracts from 293 cells, treated with insulin 0.01U/ml 15', using Anti-WAVE1 (Ab-125) Antibody.
Protocol Diagram - WAVE1 Cell Based ELISA Kit (CB5728) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 2: WAVE1 Cell Based ELISA Kit (CB5728)
A diagram showing the protocol for WAVE1 Cell Based ELISA Kit.
Protocol Summary - WAVE1 Cell Based ELISA Kit (CB5728) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 3: WAVE1 Cell Based ELISA Kit (CB5728)
A summary of the protocol for WAVE1 Cell Based ELISA Kit.
Western Blot - Control Antibody - WAVE1 Cell Based ELISA Kit (CB5728) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 4: Western Blot - WAVE1 Cell Based ELISA Kit (CB5728)
The mouse monoclonal antibody to GAPDH is used as a positive control inWAVE1 Cell Based ELISA Kit. Anti-GAPDH Antibody was tested for specificity by western blot with tissue lysates from human, mouse, and rat.

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