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Anti-Vimentin Anticorps (A81195)

Western Blot - Anti-Vimentin Antibody (A81195) - Antibodies.com Western Blot - Anti-Vimentin Antibody (A81195) - Antibodies.com Immunofluorescence - Anti-Vimentin Antibody (A81195) - Antibodies.com
Anticorps Validé par Knockout
$470
Product Datasheet

Anticorps polyclonal de lapin contre Vimentin pour WB, IHC et ICC/IF.

Qualité 100 % Garantie
Garantie de Correspond­ance des Prix

Frais de livraison
$40
Date de livraison
Livraison sous 5-8 jours ouvrables.

Téléphone
+1 (314) 370-6046
Lun - Ven, 8h - 16h AST
E-mail
orders@antibodies.com

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Anti-Vimentin Anticorps
Anticorps polyclonal de lapin contre Vimentin pour WB, ICC/IF et IHC.
Nom du produit
Anti-Vimentin Antibody
Description du produit
Rabbit polyclonal antibody to Vimentin.
Applications
WB, IHC, ICC/IF
Recommander des dilutions
WB: 1:500-1:1,000, IHC: 1:50-1:200, ICC/IF: 1:50-1:200
Reactivité
Human, Mouse, Rat
Immunogène
Recombinant fusion protein containing a sequence corresponding to amino acids 10-101 of human Vimentin (NP_003371.2).
Séquence
SYRRMFGGPGTASRPSSSRSYVTTSTRTYSLGSALRPSTSRSLYASSPGGVYATRSSAVRLRSSVPGVRLLQDSVDFSLADAINTEFKNTRT
Hôte
Rabbit
Clonalité
Polyclonal
Isotype
IgG
Conjuguer

Unconjugated

Purification
Affinity purification.
Masse moléculaire
57 kDa
Forme du produit
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.
Stockage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonymes
CTRCT30, Epididymis luminal protein 113, FLJ36605, HEL113, VIM, VIME_HUMAN
Contrôles isotype
Anticorps secondaires appropriés
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Western Blot - Anti-Vimentin Antibody (A81195) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 1: Western Blot - Anti-Vimentin Antibody (A81195)
Western blot analysis of extracts of various cell lines, using Anti-Vimentin Antibody (A81195) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Western Blot - Anti-Vimentin Antibody (A81195) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 2: Western Blot - Anti-Vimentin Antibody (A81195)
Western blot analysis of extracts from normal (control) and Vimentin knockout (KO) 293T cells, using Anti-Vimentin Antibody (A81195) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 5s.
Immunofluorescence - Anti-Vimentin Antibody (A81195) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 3: Immunofluorescence - Anti-Vimentin Antibody (A81195)
Immunofluorescence analysis of U2OS cells using Anti-Vimentin Antibody (A81195) at a dilution of 1:50 (40x lens). DAPI was used to stain the cell nuclei (blue).

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