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VE-Cadherin Cell Based Kit Elisa (A103371)

Nom du produit
VE-Cadherin Cell Based ELISA Kit
Type de test
Cell-based (qualitative)
Principe du test
This VE-Cadherin Cell Based ELISA Kit allows for the detection of VE-Cadherin and the effects that certain stimulation conditions have on VE-Cadherin expression in different cell lines. Qualitative determination of VE-Cadherin concentration is achieved by an indirect ELISA format. In essence, the VE-Cadherin is captured by Anti-VE-Cadherin Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this VE-Cadherin Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.
Plate-forme
Microplate
Méthode de détection
Colorimetric
Type d'échantillon
Adherent cells and suspension cells.
Gamme de detection
> 5000 Cells
Durée du test
4 hours 30 minutes
Reactivité
Human, Mouse
Precision
Intra-assay CV: < 8%, Inter-assay CV: < 10%
Stockage
Store at + 4°C. Stable for 6 months.
Synonymes
7B 4, 7B4, 7B4 antigen, CADH5_HUMAN, Cadherin 5, Cadherin 5 type 2, Cadherin 5, type 2 (vascular endothelium), Cadherin 5, type 2, VE cadherin (vascular epithelium), cadherin, vascular endothelial, cadherin, vascular endothelial, 1, Cadherin-5, Cadherin5, CD 144, CD144, CD144 antigen, CDH 5, CDH5, CDH5 protein, Endothelial specific cadherin, FLJ17376, OTTHUMP00000174777, Vascular endothelial cadherin, Vascular epithelium cadherin, VE Cad, VE Cadherin, VEC
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Western Blot - VE-Cadherin Cell Based ELISA Kit (CB5721) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 1: Western Blot - VE-Cadherin Cell Based ELISA Kit (CB5721)
Western blot analysis of extracts from HepG2 cells treated with Na3VO4 0.3mM 40', using Anti-VE-Cadherin (phopho Tyr731) Antibody.
Protocol Diagram - VE-Cadherin Cell Based ELISA Kit (CB5721) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 2: VE-Cadherin Cell Based ELISA Kit (CB5721)
A diagram showing the protocol for VE-Cadherin Cell Based ELISA Kit.
Protocol Summary - VE-Cadherin Cell Based ELISA Kit (CB5721) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 3: VE-Cadherin Cell Based ELISA Kit (CB5721)
A summary of the protocol for VE-Cadherin Cell Based ELISA Kit.
Western Blot - Control Antibody - VE-Cadherin Cell Based ELISA Kit (CB5721) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 4: Western Blot - VE-Cadherin Cell Based ELISA Kit (CB5721)
The mouse monoclonal antibody to GAPDH is used as a positive control inVE-Cadherin Cell Based ELISA Kit. Anti-GAPDH Antibody was tested for specificity by western blot with tissue lysates from human, mouse, and rat.
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