Anti-ULK3 Anticorps (A305005)

Name: Anti-ULK3 Antibody
Brand: Antibodies.com
SKU: A305005
Price: 500.0 USD
Availability: InStock

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$500

Anticorps polyclonal de lapin contre ULK3 pour WB et ICC/IF.

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 5-8 jours ouvrables.

Nous ContacterContact

Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanties

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Spécifications

Nom du produit

Anti-ULK3 Antibody

Description du produit

Rabbit polyclonal antibody to ULK3.

Applications

WB, ICC/IF

Recommander des dilutions

WB: 1:1,000, ICC/IF: 1:100

Reactivité

Human, Mouse, Rat

Immunogène

Synthetic peptide from the N-terminal of Human ULK3 (aa. 166-177).

Hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Conjuguer

Unconjugated

Purification

Peptide affinity purification.

Concentration

1 mg/ml

Masse moléculaire

~53 kDa

Forme du produit

Liquid

Formulation

Supplied in Phosphate Buffered Saline with 50% Glycerol and 0.09% Sodium Azide.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonymes

DKFZP434C131, FLJ90566, Serine/threonine-protein kinase ULK3, ULK3_HUMAN, unc 51 like kinase 3 (C. elegans), Unc-51-like kinase 3

Contrôles isotype

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (3)

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Figure 1: Western Blot - Anti-ULK3 Antibody (A305005)

Western blot analysis of rat brain cell lysates showing detection of 53.4 kDa ULK3 protein using Anti-ULK3 Antibody (A305005) at 1:1,000 for 1 hour at room temperature. Lane 1: Molecular Weight Ladder (MW). Lane 2: rat brain cell lysates. Load: 15 µg. Block: 5% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Rabbit HRP at 1:2000 for 60 minutes at room temperature. Color Development: ECL solution for 6 min in room temperature. Predicted/Observed Size: 53.4 kDa.

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Figure 2: Immunofluorescence - Anti-ULK3 Antibody (A305005)

Immunocytochemistry/Immunofluorescence analysis of human neuroblastoma cell line (SK-N-BE, fixed in 4% formaldehyde for 15 min at room temperature, using Anti-ULK3 Antibody (A305005), at 1:100 for 60 minutes at room temperature. The secondary antibody used was Goat Anti-Rabbit ATTO 488 at 1:200 for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5,000 for 60 minutes at room temperature, 5 minutes at room temperature. Localization: Cytoplasm. Magnification: 60X.(A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ULK3 Antibody (D) Composite.

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Figure 3: Western Blot - Anti-ULK3 Antibody (A305005)

Western blot analysis of human lung carcinoma epithelial cell line (A549) lysate showing detection of 53.4 kDa ULK3 protein using Anti-ULK3 Antibody (A305005) at 1:1,000 for 16 hours at 4°C. Lane 1: Molecular Weight Ladder (MW). Lane 2: human A549 cell lysates. Load: 15 µg. Block: 2% BSA and 2% Skim Milk in 1X TBST. The secondary antibody used was Goat Anti-Rabbit IgG: HRP at 1:2000 for 60 minutes at room temperature. Color Development: ECL solution for 6 min in room temperature. Predicted/Observed Size: 53.4 kDa. Other Band(s): ~25 kDa in all lysates.