This antibody detects ubiquitined proteins, including poly-ubiquitinated proteins (K29-, K48-, and K63-linked mono- and polyubiquitinylated proteins). Does not detect free ubiquitin.
Applications
WB, ELISA
Recommander des dilutions
WB: 1:1,000, ELISA: 1:1,000
Reactivité
Species Independent
Immunogène
Human Ubiquitin conjugated lysozyme.
Hôte
Rabbit
Clonalité
Monoclonal
Clone
RGL3R
Isotype
IgG
Conjuguer
Unconjugated
Purification
Protein A purification.
Concentration
1 mg/ml
Forme du produit
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 50% Glycerol and 0.09% Sodium Azide.
Stockage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Figure 1: Western Blot - Anti-Ubiquitin Antibody [RGL3R] (A305235)
Western blot analysis of Purified poly-ubiquitin chains showing detection of Multiple Ubiquitin protein using Anti-Ubiquitin Antibody [RGL3R] (A305235) at 1:1,000 for 2 hours at room temperature. Lane 1: Molecular Weight Ladder (MW). Lane 2: K63 Poly Ubiquitin. Lane 3: K48 Poly Ubiquitin. Load: 2 µg. Block: 5% Skim Milk powder in TBST. The secondary antibody used was Goat Anti-Rabbit IgG:HRP at 1:4,000 for 1 hour at room temperature. Color Development: ECL solution for 5 minutes in room temperature. Predicted/Observed Size: Multiple.
Produits alternatifs à Anti-Ubiquitin Anticorps [RGL3R] (A305235)
![Western Blot - Anti-Ubiquitin Antibody [RGL3R] (A305235) - Antibodies.com](https://cdn.antibodies.com/image/catalog/305/A305235_1.png?profile=product_top)
![Western Blot - Anti-Ubiquitin Antibody [RGL3R] (A305235) - Antibodies.com](https://cdn.antibodies.com/image/catalog/305/A305235_1.png?profile=product_top_thumb)
![Western Blot - Anti-Ubiquitin Antibody [RGL3R] (A305235) - Antibodies.com](https://cdn.antibodies.com/image/catalog/305/A305235_1.png?profile=product_image)
![Immunocytochemistry/Immunofluorescence - Anti-Ubiquitin Antibody [FK2] (A305218) - Antibodies.com](https://cdn.antibodies.com/image/catalog/305/A305218_1.png?profile=product_alternative)
![Western Blot - Anti-Ubiquitin Antibody [ARC50024] (A307666) - Antibodies.com](https://cdn.antibodies.com/image/catalog/307/A307666_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-Ubiquitin Antibody [UBB/1748] - BSA and Azide free (A253441) - Antibodies.com](https://cdn.antibodies.com/image/catalog/253/A253441_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-Ubiquitin Antibody [UBB/1748] (A250261) - Antibodies.com](https://cdn.antibodies.com/image/catalog/250/A250261_1.jpg?profile=product_alternative)
![Western Blot - Anti-Ubiquitin Antibody [5B9-B3] (A305078) - Antibodies.com](https://cdn.antibodies.com/image/catalog/305/A305078_1.png?profile=product_alternative)
![Immunocytochemistry/Immunofluorescence - Anti-Ubiquitin Antibody [6C11-B3] (A305079) - Antibodies.com](https://cdn.antibodies.com/image/catalog/305/A305079_1.png?profile=product_alternative)
![Immunohistochemistry - Anti-Ubiquitin Antibody [UBB/2122] - BSA and Azide free (A253442) - Antibodies.com](https://cdn.antibodies.com/image/catalog/253/A253442_1.jpg?profile=product_alternative)
![Immunohistochemistry - Anti-Ubiquitin Antibody [UBB/2122] (A250262) - Antibodies.com](https://cdn.antibodies.com/image/catalog/250/A250262_1.jpg?profile=product_alternative)