Anti-Ubiquitin Anticorps [ARC50024] (A307666)

Western blot analysis of extracts of various cell lines, using Anti-Ubiquitin Antibody [ARC50024] (A307666) at 1:1,000 dilution. HeLa, NIH/3T3 and C6 cell were treated by MG132(50 µM) at 37°C for 90 minutes. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 20s.

Immunohistochemistry analysis of paraffin-embedded rat kidney using Anti-Ubiquitin Antibody [ARC50024] (A307666) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded rat heart using Anti-Ubiquitin Antibody [ARC50024] (A307666) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded human liver cancer using Anti-Ubiquitin Antibody [ARC50024] (A307666) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.