This antibody recognises ubiquitin, a 8.6 kDa cytoplasmic and nuclear protein highly conserved across species. Ubiquitin functions as a protein modifier which covalently attaches to lysines as either a monomer or as a lysine-linked polymer. Attachment as a Lys-48 polymer usually leads to the target protein undergoing proteolytic degradation. Attachment as a monomer does not result in degradation and may be linked to functions including maintenance of chromatin structure, regulation of gene expression, stress response and DNA repair.Ubiquitin can be detected in both normal and diseased brain tissues including the neurofibrillary tangles of Alzheimers disease, Lewy bodies in Parkinsons disease and Pick bodies in Pick’s disease.
Applications
ELISA
Recommander des dilutions
ELISA: 1:100 - 1:2,000
Reactivité
Bovine
Immunogène
Native ubiquitin, purified from bovine erythrocytes to a purity >90% by SDS-PAGE.
Hôte
Sheep
Clonalité
Polyclonal
Isotype
IgG
Conjuguer
Unconjugated
Purification
Protein G affinity chromatography.
Concentration
5 mg/ml
Forme du produit
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.
Stockage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
Notes générales
Sheep anti Bovine ubiquitin antibody recognizes ubiquitin, a 8.6 kDa cytoplasmic and nuclear protein highly conserved across species. Ubiquitin functions as a protein modifier which covalently attaches to lysines as either a monomer or as a lysine-linked polymer. Attachment as a Lys-48 polymer usually leads to the target protein undergoing proteolytic degradation. Attachment as a monomer does not result in degradation and may be linked to functions including maintenance of chromatin structure, regulation of gene expression, stress response and DNA repair.Ubiquitin can be detected in both normal and diseased brain tissues including the neurofibrillary tangles of Alzheimers disease, Lewy bodies in Parkinsons disease and Pick bodies in Pick’s disease.