Anti-Ubiquitin Anticorps [5B9-B3] (A305078)

Western blot analysis of human cell lysates showing detection of Ubiquitin protein using Anti-Ubiquitin Antibody [5B9-B3] (A305078) at 1:1,000 for 2 hours at room temperature. Load: 15 µg. Block: 1.5% BSA for 30 minutes at room temperature. The secondary antibody used was Sheep Anti-Mouse IgG: HRP for 1 hour at room temperature.

Immunocytochemistry/Immunofluorescence analysis of human cervical cancer cell line (HeLa), fixed in 2% formaldehyde for 20 minutes at room temperature, using Anti-Ubiquitin Antibody [5B9-B3] (A305078), at 1:100 for 12 hours at 4°C. The secondary antibody used was FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at room temperature. Localization: Diffuse nuclear and cytoplasmic staining. Magnification: 20x.(A) DAPI (blue) nuclear stain. (B) Anti-Ubiquitin Antibody. (C) Composite.

Immunocytochemistry/Immunofluorescence analysis of human cervical cancer cell line (HeLa), fixed in 2% formaldehyde for 20 minutes at room temperature, using Anti-Ubiquitin Antibody [5B9-B3] (A305078), at 1:100 for 12 hours at 4°C. The secondary antibody used was FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at room temperature. Localization: Diffuse nuclear and cytoplasmic staining. Magnification: 100x.(A) DAPI (blue) nuclear stain. (B) Anti-Ubiquitin Antibody. (C) Composite.