Anti-Tubulin Antibody (A80592)

Name: Anti-Tubulin Antibody
Brand: Antibodies.com
SKU: A80592
Price: 280.0 USD
Availability: InStock

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$280

Rabbit polyclonal antibody to Tubulin for WB, IHC and ICC/IF.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Tags:

Microtubule Marker

Specifications

Name

Anti-Tubulin Antibody

Description

Rabbit polyclonal antibody to Tubulin.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:100-1:500, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence within amino acids 1-80 of human Alpha-tubulin (ubiquitous) chain (NP_006073.2).

Sequence

MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVIDEVRT

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

52 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (6)

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Western Blot - Anti-Tubulin Antibody (A80592)

Western blot analysis of extracts of various cell lines, using Anti-Tubulin Antibody (A80592) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 1s.

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Immunohistochemistry - Anti-Tubulin Antibody (A80592)

Immunohistochemistry analysis of paraffin-embedded mouse spleen using Anti-Tubulin Antibody (A80592)Immunohistochemistry analysis of paraffin-embedded Mouse spleen using Alpha-tubulin (ubiquitous) chain antibody (AC024) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Immunohistochemistry - Anti-Tubulin Antibody (A80592)

Immunohistochemistry analysis of paraffin-embedded rat kidney using Anti-Tubulin Antibody (A80592)Immunohistochemistry analysis of paraffin-embedded Rat kidney using Alpha-tubulin (ubiquitous) chain antibody (AC024) at a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Immunofluorescence - Anti-Tubulin Antibody (A80592)

Immunofluorescence analysis of NIH/3T3 cells using Anti-Tubulin Antibody (A80592) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).