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TRIP13 Cell Based Kit Elisa (A103028)

Nom du produit
TRIP13 Cell Based ELISA Kit
Type de test
Cell-based (qualitative)
Principe du test
This TRIP13 Cell Based ELISA Kit allows for the detection of TRIP13 and the effects that certain stimulation conditions have on TRIP13 expression in different cell lines. Qualitative determination of TRIP13 concentration is achieved by an indirect ELISA format. In essence, the TRIP13 is captured by Anti-TRIP13 Antibodies which in turn are detected by HRP-conjugated secondary antibodies. Through this binding, the HRP enzyme (conjugated to the secondary antibody) can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of this TRIP13 Cell Based ELISA Kit, multiple normalization methods are described: 1) Anti-GAPDH Antibody is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted.
Plate-forme
Microplate
Méthode de détection
Colorimetric
Type d'échantillon
Adherent cells and suspension cells.
Gamme de detection
> 5000 Cells
Durée du test
4 hours 30 minutes
Reactivité
Human, Mouse, Rat
Precision
Intra-assay CV: < 8%, Inter-assay CV: < 10%
Stockage
Store at + 4°C. Stable for 6 months.
Synonymes
16E1-BP, 16E1BP, Homo sapiens HPV16 E1 protein binding protein mRNA complete cds, HPV16 E1 protein binding protein, HPV16 E1 protein-binding protein, Human papillomavirus type 16 E1 protein binding protein, Human papillomavirus type 16 E1 protein-binding protein, Pachytene checkpoint protein 2 homolog, PCH2, Thyroid hormone receptor interactor 13, Thyroid receptor interacting protein 13, Thyroid receptor-interacting protein 13, TR-interacting protein 13, TRIP-13, TRP13_HUMAN
Avertissement
Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.
Western Blot - TRIP13 Cell Based ELISA Kit (CB6025) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 1: Western Blot - TRIP13 Cell Based ELISA Kit (CB6025)
Western blot analysis of extracts from LOVO/NIH-3T3 cells, using Anti-TRIP13 Antibody. The lane on the right is treated with the synthesized peptide.
Protocol Diagram - TRIP13 Cell Based ELISA Kit (CB6025) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 2: TRIP13 Cell Based ELISA Kit (CB6025)
A diagram showing the protocol for TRIP13 Cell Based ELISA Kit.
Protocol Summary - TRIP13 Cell Based ELISA Kit (CB6025) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 3: TRIP13 Cell Based ELISA Kit (CB6025)
A summary of the protocol for TRIP13 Cell Based ELISA Kit.
Western Blot - Control Antibody - TRIP13 Cell Based ELISA Kit (CB6025) - Antibodies.comCliquez pour ouvrir le point de vue élargi
Figure 4: Western Blot - TRIP13 Cell Based ELISA Kit (CB6025)
The mouse monoclonal antibody to GAPDH is used as a positive control inTRIP13 Cell Based ELISA Kit. Anti-GAPDH Antibody was tested for specificity by western blot with tissue lysates from human, mouse, and rat.
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