Anti-TRIM29 Anticorps (A84758)

Name: Anti-TRIM29 Antibody
Brand: Antibodies.com
SKU: A84758
Price: 480.0 USD
Availability: InStock

Ce produit est identique à Abcam (ab99486). Découvrez plus pour moins cher.

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$480

Anticorps polyclonal de chèvre contre TRIM29 pour ELISA, WB, IF, Flow Cytometry et IHC.

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 3-5 jours ouvrables.

Nous ContacterContact

Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanties

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Spécifications

Nom du produit

Anti-TRIM29 Antibody

Description du produit

Goat polyclonal antibody to TRIM29.

Applications

ELISA, WB, IF, Flow Cytometry, IHC

Recommander des dilutions

ELISA: 1:128,000, WB: 0.1-1µg/ml, IHC: 3.75µg/ml, IF: 10µg/ml

Reactivité

Human

Immunogène

Synthetic peptide corresponding to the internal region of human TRIM29.

Séquence

C-EKDRIKSFTTNE

Hôte

Goat

Clonalité

Polyclonal

Isotype

IgG

Conjuguer

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Masse moléculaire

37 kDa

MW prédit

37.3kDa

Forme du produit

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonymes

Ataxia telangiectasia group D associated protein, Ataxia telangiectasia group D-associated protein, ATDC, FLJ36085, TRI29_HUMAN, TRIM 29, Tripartite motif containing 29, Tripartite motif containing protein 29, Tripartite motif protein 29, Tripartite motif protein TRIM29, Tripartite motif-containing protein 29

Contrôles isotype

Goat IgG (A121671)

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (5)

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Figure 1: Western Blot - Anti-TRIM29 Antibody (A84758)

TRIM29 expression in Peripheral Blood Mononucleocyte (A) and HeLa (B) cell lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary incubation was performed with Anti-TRIM29 Antibody (A84758) at 1µg/ml (A) or 0.1 µg/ml (B) and detected by chemiluminescence.

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Figure 2: Immunofluorescence - Anti-TRIM29 Antibody (A84758)

TRIM29 expression in A431 cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-TRIM29 Antibody (A84758) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 4µg/ml. Cytoplasmic and intermediate filament staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 4µg/ml.

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Figure 3: Immunofluorescence - Anti-TRIM29 Antibody (A84758)

TRIM29 expression in U2OS cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-TRIM29 Antibody (A84758) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 4µg/ml. Nuclear staining shown and nuclei were stained with DAPI (blue). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 4µg/ml.

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Figure 4: Flow Cytometry - Anti-TRIM29 Antibody (A84758)

TRIM29 expression in A431 cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-TRIM29 Antibody (A84758) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.