Anti-TRIM21 Anticorps (A83914)

Name: Anti-TRIM21 Antibody
Brand: Antibodies.com
SKU: A83914
Price: 480.0 USD
Availability: InStock

Ce produit est identique à Abcam (ab4369). Découvrez plus pour moins cher.

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$480

Anticorps polyclonal de chèvre contre TRIM21 pour ELISA, WB, IF et Flow Cytometry.

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Livraison

Frais de livraison

$40

Date de livraison

Livraison sous 3-5 jours ouvrables.

Nous ContacterContact

Téléphone

+1 (314) 370-6046

E-mail

orders@antibodies.com

Garanties

Qualité 100 % Garantie

Garantie de Correspondance des Prix

Spécifications

Nom du produit

Anti-TRIM21 Antibody

Description du produit

Goat polyclonal antibody to TRIM21.

Applications

ELISA, WB, IF, Flow Cytometry

Recommander des dilutions

ELISA: 1:16,000, WB: 0.2-1µg/ml, IF: 10µg/ml, Flow Cytometry: 10µg/ml

Reactivité

Human

Immunogène

Synthetic peptide corresponding to the C terminal region of human TRIM21.

Séquence

CPLNIGSQGSTDY

Hôte

Goat

Clonalité

Polyclonal

Isotype

IgG

Conjuguer

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Masse moléculaire

60 kDa

Forme du produit

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Stockage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonymes

52 kDa ribonucleoprotein autoantigen Ro/SS-A, 52 kDa Ro protein, 52kD Ro/SSA autoantigen, Autoantigen Ro/SSA, 52-KD, E3 ubiquitin-protein ligase TRIM21, RING finger protein 81, RNF81, Ro 52, Ro(SS-A), Ro52, RO52_HUMAN, Sicca syndrome antigen A, Sjoegren syndrome type A antigen, Sjogren syndrome antigen A1, Sjogren syndrome type A antigen, SS-A, SSA, SSA1: Sjogren syndrome antigen A1 (52kDa ribonucleoprotein autoantigen SS-A/Ro), Tripartite motif protein TRIM21, Tripartite motif-containing 21, Tripartite motif-containing protein 21

Contrôles isotype

Goat IgG (A121671)

Anticorps secondaires appropriés

Avertissement

Ce produit est uniquement destiné à la recherche. Il n'est pas destiné à un usage diagnostique ou thérapeutique.

Images (3)

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Figure 1: Western Blot - Anti-TRIM21 Antibody (A83914)

TRIM21 expression in HEK293 cell lysate analyzed by western blot. Cells were transfected to overexpress MYC tagged TRIM21 an lysed in RIPA buffer. Primary incubation was performed on 10µg protein per lane with Anti-TRIM21 Antibody (A83914) at 1µg/ml or Anti-MYC at 1:1000 dilution (Lane C) and detected with chemiluminescence. Control: Mock-transfected HEK293 cells incubated with Anti-TRIM21 Antibody (A83914) at 1mg/ml (Lane B).

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Figure 2: Immunofluorescence - Anti-TRIM21 Antibody (A83914)

TRIM21 expression in U2OS cells analyzed by immunofluorescence. Cells were permeabilized with 0.15% Triton. Staining was performed with Anti-TRIM21 Antibody (A83914) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 2µg/ml. Some cytoplasmic and strong nuclear staining shown and nuclei were stained with DAPI (blue) while actin filaments were stained with phalloidin (red). Negative control: Goat IgG Isotype Control at 10µg/ml followed by Alexa Fluor 488 secondary antibody at 2µg/ml.

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Figure 3: Flow Cytometry - Anti-TRIM21 Antibody (A83914)

TRIM21 expression in HeLa cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-TRIM21 Antibody (A83914) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.