Rat monoclonal [YTA74.4] antibody to Transferrin Receptor (FITC).
Spécificité
This antibody recognises the mouse Transferrin receptor protein 1 also known as CD71 or TfR1. CD71 is a 763 amino acid glycoprotein homodimer of ~95 kDa subunits. CD71 is expressed by dividing cells, and functions as a transferrin receptor mediating uptake of iron.Rat anti Mouse CD71 antibody, clone YTA74.4 blocks the binding of R17 217.1.3. and R17 208.2 anti-TFR monoclonal antibodies.
Applications
Flow Cytometry
Recommander des dilutions
Flow Cytometry: Neat, Use 10uµ of the suggested working dilution to label 106 cells in 100µl. The Fc region of monoclonal antibodies may bind to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR ( BUF041A/B).
Reactivité
Mouse
Immunogène
Concanavilin A activated mouse spleen cells.
Hôte
Rat
Clonalité
Monoclonal
Clone
YTA74.4
Isotype
IgG2a
Conjuguer
FITC
Excitation: 490nm, Emission: 525nm
Purification
Ion exchange chromatography of tissue culture supernatant.
Concentration
100 µg/ml
Forme du produit
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.
Stockage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Notes générales
Rat anti Mouse CD71 antibody, clone YTA74.4 recognizes the mouse Transferrin receptor protein 1 also known as CD71 or TfR1. CD71 is a 763 amino acid glycoprotein homodimer of ~95 kDa subunits. CD71 is expressed by dividing cells, and functions as a transferrin receptor mediating uptake of iron.Rat anti Mouse CD71 antibody, clone YTA74.4 blocks the binding of R17 217.1.3. and R17 208.2 anti-TFR monoclonal antibodies (Trowbridge et al. 1982).